DSMZ 708 . DESULFORHABDUS AMNIGENA MEDIUM

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Equipment needed: Autoclave Filter Gassing station Hungate tubes

Final gas composition: 80 % N2 20 % CO2 read more

Final pH: 7.2 - 7.6

Select a strain (optional):

ml
Compound Amount Unit
Na2HPO4 x 2 H2O 0.53 g
KH2PO4 0.41 g
NH4Cl 0.30 g
CaCl2 x 2 H2O 0.11 g
MgCl2 x 6 H2O 0.10 g
Na2SO4 2.80 g
NaCl 0.30 g
Trace element solution SL-10 1.00 ml
Selenite-tungstate solution 1.00 ml
Sodium resazurin
(0.1% w/v)
0.50 ml
Na2CO3 1.50 g
Wolin's vitamin solution (10x) 1.00 ml
Seven vitamins solution 1.00 ml
Na-propionate 2.00 g
Na2S x 9 H2O 0.50 g
Distilled water 1000.00 ml
1 Dissolve ingredients except carbonate, vitamins, propionate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, propionate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.2 - 7.6.

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%)
10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Compound Amount Unit
NaOH 0.5 g
Na2SeO3 x 5 H2O 3.0 mg
Na2WO4 x 2 H2O 4.0 mg
Distilled water 1000.0 ml