736 .
RICH MEDIUM
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* Medium for Intrasporangium sp. DSM 24459
Plus 100 µl Anaerobiersalze (salt solution of DSMZ Medium 104)
Growth conditions: aerobic
28 °C
pH 7.20
Equipment needed:
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone
(Difco) |
10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Casamino acids
(Difco) |
5.00 | g | ||
|
|
Meat extract
(Difco) |
2.00 | g | ||
|
|
Malt extract
(Difco) |
5.00 | g | ||
|
|
Glycerol | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
Tween 80 | 0.05 | g | ||
|
|
Agar | 20.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Final pH is 7.2. | |||||