780 .
MIDDLEBROOK MEDIUM WITH MYCOBACTIN
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Mycobactine J | 2 | mg | ||
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Absolute ethanol | 4 | ml | ||
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Medium 645 | 1000 | ml | ||
| 1 Reconstitute the contents of one vial of 2 mg Mycobactine J with 2 ml absolute ethanol (analytical grade). Shake carefully so that the product is completely dissolved. Pour the contents of the bottle into 1 litre of medium 645 kept melted in a water bath at +56°C, taking care to pour out all of the product. Rinse out the vial that contained the Mycobactine J with an additional 2 ml of absolute ethanol and pour into the culture medium. Mix the Mycobactine J alcohol solution and the culture medium very thoroughly. | |||||
Main sol. 645 #
905
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Bacto Middlebrook 7H10 agar
(Difco 262710) |
19 | g | ||
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Glycerol | 5 | ml | ||
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Distilled water | 900 | ml | ||
| 1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly. | |||||
| 2 If you can´t order the commercial media then prepare the media in this way: | |||||