783 .
HYDROGENOTHERMUS HIRSCHII MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 7.00 | g | ||
|
|
MgCl2 x 6 H2O | 5.50 | g | ||
|
|
KCl | 0.65 | g | ||
|
|
NaBr | 0.10 | g | ||
|
|
NaHCO3 | 2.00 | g | ||
|
|
NH4Cl | 0.15 | g | ||
|
|
K2HPO4 | 0.15 | g | ||
|
|
CaCl2 x 2 H2O | 0.50 | g | ||
|
|
Modified Wolin's mineral solution II | 10.00 | ml | ||
|
|
Sulfur
(powdered) |
0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare the medium aerobically and adjust pH to 7.0 using H2SO4. Fill 20 ml medium into 100 ml serum bottles under H2/CO2 (80/20) gas and seal with a rubber stopper. Steam medium for 3 hours on each of 3 successive days. Before use add 100 µl of a sterile CaCO3 solution (10%). Give an atmosphere of H2/CO2 with an overpressure of two atmospheres and 20 ml filter-sterilized air. Incubate under shaking at 60°C. | |||||
Modified Wolin's mineral solution II #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.500 | g | ||
|
|
MgSO4 x 7 H2O | 3.000 | g | ||
|
|
MnSO4 x H2O | 0.500 | g | ||
|
|
NaCl | 1.000 | g | ||
|
|
FeSO4 x 7 H2O | 0.100 | g | ||
|
|
CoSO4 x 7 H2O | 0.180 | g | ||
|
|
CaCl2 x 2 H2O | 0.100 | g | ||
|
|
ZnSO4 x 7 H2O | 0.180 | g | ||
|
|
CuSO4 x 5 H2O | 0.010 | g | ||
|
|
AlK(SO4)2 x 12 H2O | 0.020 | g | ||
|
|
H3BO3 | 0.010 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.010 | g | ||
|
|
NiCl2 x 6 H2O | 0.025 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.300 | mg | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||