791 .
PORPHYROBACTER TEPIDARIUS MEDIUM
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Selected strain: Tepidimonas sp. DSM 14591
This strain uses the standard medium without modifications.
Growth conditions: 50 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Na glutamate | 0.5 | g/l | ||
|
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Sodium succinate | 0.5 | g/l | ||
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Sodium acetate | 0.5 | g/l | ||
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Yeast extract | 0.5 | g/l | ||
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Casamino acids | 0.5 | g/l | ||
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(NH4)2SO4 | 0.5 | g/l | ||
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Sodium thiosulphate | 0.5 | g/l | ||
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Basal salts solution | 5.0 | ml | ||
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Phosphate solution | 5.0 | ml | ||
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Vitamin solution | 1.0 | ml | ||
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Distilled water | 989.0 | ml | ||
| 1 Final pH 7.5 | |||||
| 2 Add the vitamin solution, aseptically to the cooled, autoclaved medium. | |||||
Basal salts solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na3-EDTA | 4.53 | g/l |
|
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FeSO4 x 7 H2O | 1.11 | g/l |
|
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MgSO4 x 7 H2O | 24.65 | g/l |
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CaCl2 x 2 H2O | 2.94 | g/l |
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NaCl | 23.40 | g/l |
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MnSO4 x 4 H2O | 112.00 | mg/l |
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ZnSO4 x 7 H2O | 29.00 | mg/l |
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Co(NO3)2 x 6 H2O | 29.00 | mg/l |
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CuSO4 x 5 H2O | 25.00 | mg/l |
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Na2Mo4 x 2 H2O | 24.00 | mg/l |
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H3BO3 | 31.00 | mg/l |
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Distilled water | 1000.00 | ml |
Vitamin solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nicotinic acid | 100 | mg | ||
|
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Thiamine | 100 | mg | ||
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Biotin | 5 | mg | ||
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p-Aminobenzoic acid | 50 | mg | ||
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Vitamin B12 | 1 | mg | ||
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Calcium pantothenate | 50 | mg | ||
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Pyridoxine hydrochloride | 50 | mg | ||
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Folic acid | 50 | mg | ||
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Na3-EDTA | 200 | mg | ||
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Distilled water | 100 | ml | ||
| 1 Filter sterilize. | |||||