MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 835 . DESULFUROMONAS CHLOROETHENICA MEDIUM

What is this page?
Equipment needed: Autoclave Filter Gassing station Hungate tubes
Final gas composition: 80 % N2 20 % CO2 read more
Final pH: 7.0 - 7.2
Select a strain (optional):

ml
Compound Amount Unit
Solution A 921 ml
Solution B 35 ml
Solution C 25 ml
Solution D 10 ml
Solution E 1 ml
Solution F 10 ml
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 -45 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions D and F are autoclaved under 100% N2 gas atmosphere. Solutions C and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 - 7.2.

Solution A #

921
Compound Amount Unit
NH4Cl 0.40 g
KH2PO4 0.50 g
MgCl2 x 6 H2O 0.30 g
NaCl 0.40 g
CaCl2 x 2 H2O 10.00 mg
Trace element solution SL-10 1.00 ml
Na-acetate 0.41 g
Sodium resazurin
(0.1% w/v) 		0.50 ml
Distilled water 920.00 ml

Solution B #

35
Compound Amount Unit
Na2CO3 1.75 g
Distilled water 35.00 ml

Solution C #

25
Compound Amount Unit
Na2-fumarate 3.2 g
Distilled water 25.0 ml

Solution D #

10
Compound Amount Unit
L-Cysteine HCl x H2O 0.25 g
Distilled water 10.00 ml

Solution E #

1
Compound Amount Unit
Wolin's vitamin solution (10x) 1 ml

Solution F #

10
Compound Amount Unit
Na2S x 9 H2O 0.25 g
Distilled water 10.00 ml

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%) 		10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution (10x) #

1000
Compound Amount Unit
Biotin 20 mg
Folic acid 20 mg
Pyridoxine hydrochloride 100 mg
Thiamine HCl 50 mg
Riboflavin 50 mg
Nicotinic acid 50 mg
Calcium D-(+)-pantothenate 50 mg
Vitamin B12 1 mg
p-Aminobenzoic acid 50 mg
(DL)-alpha-Lipoic acid 50 mg
Distilled water 1000 ml