DSMZ 860 . ANAEROCOLUMNA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
* Modified for Clostridium thiosulfatireducens DSM 13105

Replace fructose with 2.5 g/l Na2S2O3 x 5 H2O and adjust pH of complete medium to 7.4.

Equipment needed: Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.998 18.658
K2HPO4 0.3 g 0.299 1.719
KH2PO4 0.3 g 0.299 2.2
MgCl2 x 6 H2O 0.2 g 0.2 0.982
CaCl2 x 2 H2O 0.1 g 0.1 0.679
KCl 0.1 g 0.1 1.339
NaCl 0.6 g 0.599 10.246
Yeast extract 1.0 g 0.998 -
Trace element solution SL-10 1.5 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
Na2CO3 1.5 g 1.497 14.124
D-Fructose* 4.0 g 3.992 22.158
L-Cysteine HCl x H2O 0.5 g 0.499 2.841
Na2S x 9 H2O 0.3 g 0.299 1.247
Na2S2O3 x 5 H2O* 2.5 g - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients except carbonate, fructose, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 07.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: * 7.4
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 6
Strains with modifications: 5
Clostridium thiosulfatireducens DSM 13105

  D-Fructose
  7.4
  2.5 g Na2S2O3 x 5 H2O

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2.495 10.053
Na2CO3 1.497 14.124
NH4Cl 0.998 18.658
Yeast extract 0.998 -
NaCl 0.599 10.246
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Cultivation metadata from 6 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 7 strains
oxygen anaerobe 6 strains
temperature mesophilic 6 strains
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