DSMZ 860 . ANAEROCOLUMNA MEDIUM

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Equipment needed: Autoclave Gassing station Hungate tubes

Final gas composition: 80 % N2 20 % CO2 read more

Final pH: 7.0 - 7.2

Select a strain (optional):

ml
Compound Amount Unit
NH4Cl 1.0 g
K2HPO4 0.3 g
KH2PO4 0.3 g
MgCl2 x 6 H2O 0.2 g
CaCl2 x 2 H2O 0.1 g
KCl 0.1 g
NaCl 0.6 g
Yeast extract 1.0 g
Trace element solution SL-10 1.5 ml
Sodium resazurin
(0.1% w/v)
0.5 ml
Na2CO3 1.5 g
D-Fructose 4.0 g
L-Cysteine HCl x H2O 0.5 g
Na2S x 9 H2O 0.3 g
Distilled water 1000.0 ml
1 Dissolve ingredients except carbonate, fructose, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.0 - 7.2, if necessary.

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%)
10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.