887 .
OS MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 256.00 | mg | ||
|
|
KH2PO4 | 1.70 | mg | ||
|
|
CaCl2 x 2 H2O | 0.80 | mg | ||
|
|
H3BO3 | 10.30 | mg | ||
|
|
Na2SO4 | 23.00 | mg | ||
|
|
NaNO3 | 0.30 | mg | ||
|
|
KCl | 15.00 | mg | ||
|
|
FeCl3 x 6 H2O | 0.10 | mg | ||
|
|
MnSO4 x 4 H2O | 0.06 | mg | ||
|
|
Modified Wolin's mineral solution II | 10.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Sparge medium with 100% N2 gas mixture for 30 min to make anoxic. | |||||
| 2 Adjust pH to 7.0 with H2SO4. | |||||
| 3 Fill 10 ml medium into 100 ml Serum bottles under a stream of H2/CO2 gas and seal with a rubber stopper. | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
| 5 After autoclaving add the following (e.g., from sterile stock solutions) | |||||
|
|
Na2S2O3 x 5 H2O
(10% in H2O) |
1.00 | g/l | ||
|
|
NaHCO3
(5 % in H2O) |
1.00 | g/l | ||
| 6 Adjust the gas atmosphere strain specifically. | |||||
Modified Wolin's mineral solution II #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.500 | g | ||
|
|
MgSO4 x 7 H2O | 3.000 | g | ||
|
|
MnSO4 x H2O | 0.500 | g | ||
|
|
NaCl | 1.000 | g | ||
|
|
FeSO4 x 7 H2O | 0.100 | g | ||
|
|
CoSO4 x 7 H2O | 0.180 | g | ||
|
|
CaCl2 x 2 H2O | 0.100 | g | ||
|
|
ZnSO4 x 7 H2O | 0.180 | g | ||
|
|
CuSO4 x 5 H2O | 0.010 | g | ||
|
|
AlK(SO4)2 x 12 H2O | 0.020 | g | ||
|
|
H3BO3 | 0.010 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.010 | g | ||
|
|
NiCl2 x 6 H2O | 0.025 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.300 | mg | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||