911 .
MIDDLEBROCK 7H11 AGAR
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Equipment needed:
Final pH:
6.4 - 6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto Middelbrook 7H11 agar (Difco 283810) | 21 | g | ||
|
|
Glycerol | 5 | ml | ||
|
|
Distilled water | 900 | ml | ||
|
|
Bacto Middelbrook OADC enrichment (Difco 212240): | 100 | ml | ||
| 1 Bacto Middelbrook OADC enrichment (Difco 212240): | |||||
| 2 Sterilize for 10 min by 121°C cool to 50 - 55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions and mix thoroughly. | |||||
| 3 Final pH at 25°C 6.6 ± 0.2. | |||||
Bacto Middelbrook 7H11 agar (Difco 283810) #
900
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Pancreatic digest of casein | 1.0 | g |
|
|
(NH4)2SO4 | 0.5 | g |
|
|
KH2PO4 | 1.5 | g |
|
|
Na2HPO4 | 1.5 | g |
|
|
Sodium citrate | 0.4 | g |
|
|
MgSO4 | 50.0 | mg |
|
|
L-Glutamic acid | 0.5 | g |
|
|
Ferric ammonium citrate | 40.0 | mg |
|
|
Pyridoxine | 1.0 | mg |
|
|
Biotin | 0.5 | mg |
|
|
Malachite green | 1.0 | mg |
|
|
Agar
(Bacto ) |
15.0 | g |
|
|
Distilled water | 900.0 | ml |
Bacto Middelbrook OADC enrichment (Difco 212240): #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Oleic acid | 50 | mg |
|
|
Bovine albumin
(albumin fraction V) |
5 | g |
|
|
Dextrose | 2 | g |
|
|
Catalase
(beef) |
4 | mg |
|
|
NaCl | 850 | mg |
|
|
Distilled water | 100 | ml |