923 .
ALKALIBACTERIUM OLIVAPOVLITICUS
What is this page?
Selected strain: Bacillus sp. DSM 12918
This strain uses the standard medium without modifications.
Growth conditions: 25 °C
Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na glutamate
(Sigma) |
10.000 | g | ||
|
|
Yeast extract
(Oxoid) |
5.000 | g | ||
|
|
Na2CO3 | 10.000 | g | ||
|
|
K2HPO4 | 0.150 | g | ||
|
|
MgSO4 x 7 H2O | 0.025 | g | ||
|
|
(NH4)2SO4 | 1.000 | g | ||
|
|
Distilled water | 1000.000 | ml | ||
|
|
Agar , for solid medium | 20.000 | g | ||
| 1 We recommend that liquid media be made by autoclaving the Na2CO3 separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na2CO3 otherwise the agar will be hydrolysed. When mixing the ingredients for solid media cool all components to about 55°C otherwise hydrolysis of the agar may occur. | |||||