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DSMZ

DSMZ 951 . METHYLOPHAGA SULFIDOVORANS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methylophaga sulfidovorans DSM 11578

This strain uses the standard medium without modifications.

Growth conditions: 25 °C

Main sol. 951 #

ml
Equipment needed: Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.00 g 15 256.674
(NH4)2SO4 0.50 g 0.5 3.784
CaCl2 x 6 H2O 0.33 g 0.33 1.506
KCl 0.20 g 0.2 2.683
MgSO4 x 7 H2O 1.00 g 1 4.057
KH2PO4 0.02 g 0.02 0.147
Na2CO3 2.00 g 2 18.87
FeSO4 x 7 H2O 1.00 mg 1.0e-3 0.004
Trace element solution SL10 1.00 ml - -
Vitamin solution 1.00 ml - -
Methanol 10.00 ml 7.92 247.176
Agar 15.00 g 15 44.599
Distilled water 998.00 ml - -
1 Prepare the medium without methanol and the vitamin solution. Adjust pH to 7.5 (±0.3) with 2 N HCl. Liquid media is prepared in either Hungate tubes (5ml medium) or 100ml serum bottles (10ml medium) sealed with rubber stoppers.
2 Sterilize by autoclaving. After autoclaving add the methanol (filter sterilised) and the vitamin solution (filter sterilised) from sterile stock solutions.

Trace element solution SL10 #

1005
Solution occurs in 2 other media (first defined in Medium 951).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(concentrated) 		5.000 ml - -
FeCl2 x 4 H2O 1.500 g 1.493 7.507
ZnCl2 0.070 g 0.07 0.511
MnCl2 x 4 H2O 0.200 g 0.199 1.006
H3BO3 6.000 mg 0.006 0.097
CoCl2 x 6 H2O 0.190 g 0.189 1.456
CuCl2 x 2 H2O 2.000 mg 0.002 0.012
NiCl2 x 6 H2O 0.024 g 0.024 0.184
Na2MoO4 x 2 H2O 0.036 g 0.036 0.148
Distilled water 1000.000 ml - -

Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Thiamine 100 mg 0.1 0.377
p-Aminobenzoic acid 100 mg 0.1 0.729
Pantothenate 50 mg 0.05 0.228
Pyridoxine hydrochloride 500 mg 0.5 2.431
Riboflavine 10 mg 0.01 0.027
Vitamin B12 10 mg 0.01 0.007
Distilled water 1000 ml - -

Metadata

Last modified: 25.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.8
Equipment needed: Filter, Hungate tubes
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Associated strains

All strains for this medium: 1
Methylophaga sulfidovorans DSM 11578

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
Agar 15 44.599
Methanol 7.92 247.176
Na2CO3 2 18.87
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain