DSMZ 968 . ANAEROBACILLUS ARSENICISELENATIS MEDIUM

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Equipment needed: Autoclave Gassing station Hungate tubes

Final gas composition: 100 % N2 read more

Final pH: 9.8

Select a strain (optional):

ml
Compound Amount Unit
(NH4)2SO4 0.10 g
MgSO4 25.00 mg
NaCl 90.00 g
K2HPO4 0.15 g
KH2PO4 0.08 g
Trace element solution SL-10 1.00 ml
Selenite-tungstate solution 1.00 ml
NaNO3 1.25 g
Na-DL-lactate 1.70 g
Yeast extract 0.20 g
Sodium resazurin
(0.1% w/v)
0.50 ml
Na2CO3 10.60 g
NaHCO3 4.20 g
L-Cysteine HCl x H2O 0.25 g
Na2S x 9 H2O 0.25 g
Distilled water 1000.00 ml
1 Dissolve ingredients (except carbonate, hydrogencarbonate and reducing agents), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and bicarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary.

Trace element solution SL-10 #

1000
Compound Amount Unit
HCl
(25%)
10.0 ml
FeCl2 x 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 x 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 x 6 H2O 190.0 mg
CuCl2 x 2 H2O 2.0 mg
NiCl2 x 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Compound Amount Unit
NaOH 0.5 g
Na2SeO3 x 5 H2O 3.0 mg
Na2WO4 x 2 H2O 4.0 mg
Distilled water 1000.0 ml