999 .
HALORHODOSPIRA MEDIUM
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Equipment needed:
Filter
Tungsten lamp
Final pH:
7.2 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
NaCl | 100.00 | g | ||
|
|
MgCl2 x 6 H2O | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 After autoclaving 20 min at 121°C, the following sterile solutions are added: | |||||
|
|
NaHCO3
(20%) |
10.00 | ml | ||
|
|
Na2S x 9 H2O
(10%) |
7.50 | ml | ||
|
|
Vitamins V7 solution (Pfennig et Trüper, 1992) | 1.00 | ml | ||
|
|
Trace element solution SL12 | 1.00 | ml | ||
| 2 The pH is adjusted to pH 7.2-7.4 For better growth of pure cultures, the growth medium should be supplemented with acetate (2 mM final concentration), succinate (1 mM final concentration) and yeast extract (0.5 g/l). Pure cultures should be incubated at 30°C and light intensity of 500 lux from a tungsten lamp (16 h light, 8 h dark). Pure cultures in liquid medium can be stored in 60 ml screw cap bottles at 4°C in the dark. | |||||
Trace element solution SL12 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Distilled water | 1000.0 | ml | ||
|
|
H3BO3 | 300.0 | mg | ||
|
|
FeSO4 x 7 H2O | 1.1 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 2 H2O | 50.0 | mg | ||
|
|
ZnCl2 | 42.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 18.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
| 1 Sterilized by filtration. | |||||
Vitamins V7 solution (Pfennig et Trüper, 1992) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Distilled water | 1000 | ml | ||
|
|
Biotine | 2 | mg | ||
|
|
p-Aminobenzoate | 10 | mg | ||
|
|
Thiamine | 10 | mg | ||
|
|
Pantothenate | 5 | mg | ||
|
|
Pyridoxamine | 50 | mg | ||
|
|
Vitamine B12 | 20 | mg | ||
|
|
Nicotinate | 20 | mg | ||
| 1 Sterilized by filtration. | |||||