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CCAP 1 . ABM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C1 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.25 g 0.25 1.892
NaNO3
(75 g/l stock solution) 		10.00 ml - -
CaCl2 x 2 H2O
(2.5 g/l stock solution) 		10.00 ml - -
MgSO4 x 7 H2O
(7.5 g/l stock solution) 		10.00 ml - -
K2HPO4 x 3 H2O
(7.5 g/l stock solution) 		10.00 ml - -
KH2PO4
(17.5 g/l stock solution) 		10.00 ml 23.4 171.951
NaCl
(2.5 g/l stock solution) 		10.00 ml - -
Trace Elements (PIV) 6.00 ml - -
1 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Vitamin B1 solution 1.00 ml - -
Vitamin B12 solution 1.00 ml - -
2 The stock solutions are those for 3N-BBM+V. Make up to 1 litre with distilled water and adjust the pH to 3.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes.

Trace Elements (PIV) #

1000
Solution occurs in 7 other media (first defined in Medium C1).
1 Ensure elements are added in the following sequence:
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.750 g 0.75 2.231
FeCl3 x 6 H2O 0.097 g 0.097 0.359
MnCl2 x 4 H2O 0.041 g 0.041 0.207
ZnCl2 0.005 g 0.005 0.037
CoCl2 x 6 H2O 0.002 g 0.002 0.015
Na2 MoO4 x 2 H2O 0.004 g 0.004 0.017
2 Once elements are dissolved autoclave at 15 psi for 15 minutes.

Vitamin B1 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine hydrochloride
(Vitamin B1) 		0.12 g 1.2 3.558
1 Filter sterile

Vitamin B12 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine
(Vitamin B12) 		0.1 g 1 0.738
1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.

Metadata

Last modified: 04.10.22
Source: CCAP
Synonyms: Acidified Bold-Basal Medium with Vitamins, modified
Medium type: Defined medium
Final pH: 3.0
Reference: https://doi.org/10.1016/j.protis.2005.04.004
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Associated strains

All strains for this medium: 3
Strains with modifications: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 23.4 171.951
(NH4)2SO4 0.25 1.892
Na2-EDTA 0.005 0.013
Thiamine hydrochloride 0.001 0.004
Cyanocobalamine 1.0e-3 7.4e-4
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