108 .
ZM/10
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Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Bacto peptone | 0.5 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Agar
(Bacto) |
15.0 | g | ||
|
|
Marine Supplement Stock | 10.0 | ml | ||
|
|
Sea water
(filtered) |
750.0 | ml | ||
|
|
Deionized water | 250.0 | ml | ||
| 1 Make up to 1 litre with 75% filtered seawater: 25% deionized water. Autoclave at 15 psi for 15 minutes. Cool to approx. 55 °C and add sterile marine supplement. | |||||
Marine Supplement Stock #
100
| 1 First prepare primary stocks for marine supplement stock: | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trace elements (x10 concentration) | 1 | ml | ||
|
|
Vitamin mix | 1 | ml | ||
|
|
Na2SeO3
(0.002 g/l stock solution) |
1 | ml | ||
| 2 Autoclave at 15 psi for 15 minutes. Once at room temperature add Primary stock 2 (Vitamin mix). | |||||
Trace elements (x10 concentration) #
200
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 8.320 | g |
|
|
FeCl3 x 6 H2O | 6.300 | g |
|
|
CuSO4 x 5 H2O | 0.020 | g |
|
|
ZnSO4 x 7 H2O | 0.044 | g |
|
|
CoCl2 x 6 H2O | 0.020 | g |
|
|
MnCl2 x 4 H2O | 0.360 | g |
|
|
Na2MoO4 x 2 H2O | 0.012 | g |
Vitamin mix #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Cyanocobalamin | 0.001 | g | ||
|
|
Biotin | 0.020 | g | ||
|
|
Thiamine HCl | 0.050 | g | ||
|
|
Calcium pantothenate | 0.050 | g | ||
|
|
Folic acid | 0.020 | g | ||
|
|
Riboflavin | 0.050 | g | ||
|
|
Nicotinamide | 0.050 | g | ||
| 1 Dissolve vitamins one at a time; adjust the pH to 7 with 1 M NaOH or 1 M HCl if necessary, to dissolve the biotin and folic acid. Filter sterilise. Dispense excess into 1 ml aliquots and freeze. | |||||