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CCAP 2 . AJS

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C2 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
JM (Jaworski´s Medium) 970 ml - -
SE2 (Soil Extract 2) 30 ml - -
1 Acidified 97:3 mixture of JM and SE2
2 See separate recipes. For approximately 1 litre of final medium, mix 970 ml JM with 30 ml SE2. Add 10 ml of concentrated H2SO4 to give a pH of approximately 1.5. Autoclave at 15 psi for 15 minutes.

JM (Jaworski´s Medium) #

1000
Solution occurs in 1 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		1 ml - -
KH2PO4
(2.48 g/200 ml stock solution) 		1 ml 2.34 17.195
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		1 ml - -
NaHCO3
(3.18 g/200 ml stock solution) 		1 ml - -
Solution 1 1 ml - -
Solution 2 1 ml - -
Solution 3 1 ml - -
NaNO3
(16.0 g/200 ml stock solution) 		1 ml - -
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		1 ml - -
Deionized water 1000 ml - -
Agar , for solid medium
(Oxoid L11) 		15 g 15 -
1 Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

SE2 (Soil Extract 2) #

660
Solution occurs in 2 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air­dried, sieved) 		105 g 159.091 -
Deionized water 660 ml - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
3 Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.

Solution 1 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeNa-EDTA 0.45 g 2.25 -
Na2-EDTA 0.45 g 2.25 6.692

Solution 2 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 0.496 g 2.48 40.104
MnCl2 x 4 H2O 0.278 g 1.39 7.024
(NH4)6Mo7O24 x 4 H2O 0.200 g 1 0.809

Solution 3 #

200
Solution occurs in 4 other media (first defined in Medium C2).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 0.008 g 0.04 0.03
Thiamine HCl 0.008 g 0.04 0.119
Biotine 0.008 g 0.04 0.164

Metadata

Last modified: 04.10.22
Source: CCAP
Synonyms: Acidified JM:SE
Medium type: Complex medium
Final pH: 1.5
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 14.55 -
Soil 4.773 -
KH2PO4 2.27 16.679
H3BO3 0.002 0.039
Na2-EDTA 0.002 0.006
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