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CCAP 27 . EG:JM

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Selected strain: Chlorochytrium sp. CCAP212/1

This strain uses the standard medium without modifications.

Equipment needed:
Final pH: n.d.
Select a strain (optional):

ml
Compound Amount Unit
EG (Euglena gracilis Medium) 500 ml
JM (Jaworski´s Medium) 500 ml
1 1:1 mixture. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.

EG (Euglena gracilis Medium) #

1000
Compound Amount Unit
Sodium acetate x 3 H2O 1 g
Lab Lemco powder
(Oxoid L29) 		1 g
Tryptone
(Oxoid L42) 		2 g
Yeast extract
(Oxoid L21) 		2 g
CaCl2
(1 g/l stock solution) 		10 ml
Deionized water 1000 ml
Agar , for solid medium
(Oxoid L11) 		15 g
1 Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

JM (Jaworski´s Medium) #

1000
Compound Amount Unit
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		1 ml
KH2PO4
(2.48 g/200 ml stock solution) 		1 ml
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		1 ml
NaHCO3
(3.18 g/200 ml stock solution) 		1 ml
Solution 1 1 ml
Solution 2 1 ml
Solution 3 1 ml
NaNO3
(16.0 g/200 ml stock solution) 		1 ml
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		1 ml
Deionized water 1000 ml
Agar , for solid medium
(Oxoid L11) 		15 g
1 Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

Solution 1 #

200
Compound Amount Unit
FeNa-EDTA 0.45 g
Na2-EDTA 0.45 g

Solution 2 #

200
Compound Amount Unit
H3BO3 0.496 g
MnCl2 x 4 H2O 0.278 g
(NH4)6Mo7O24 x 4 H2O 0.200 g

Solution 3 #

200
Compound Amount Unit
Cyanocobalamine 0.008 g
Thiamine HCl 0.008 g
Biotine 0.008 g