3 .
ANT
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Equipment needed:
Final pH:
7.6 - 7.8
Description:
Referece: Antia NJ & Kalmakoff J (1965) Fish. Res. Bd Can., Manuscr. Rep. Ser. (Oceanogr.
Limnol.) No. 203 -
Antia NJ, Cheng JY & Taylor FJR (1969) Proc. Int. Seaweed Symp. 6, 1729
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KNO3 | 0.0500 | g | ||
|
|
NaH2PO4 x 2 H2O | 0.0078 | g | ||
|
|
Tris | 1.0000 | g | ||
|
|
Glycine | 0.3000 | g | ||
|
|
Trace metals stock solution (chelated) | 2.5000 | ml | ||
|
|
Thiamine HCl | 500.0000 | μg | ||
|
|
Cyanocobalamine | 2.0000 | μg | ||
|
|
Biotine | 1.0000 | μg | ||
|
|
Natural sea water
(filtered) |
800.0000 | ml | ||
| 1 Make up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 - 7.8. | |||||
Trace metals stock solution (chelated) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA x 2 H2O | 3.2400 | g | ||
|
|
FeCl3 x 6 H2O | 1.0800 | g | ||
|
|
MnSO4 x 4 H2O | 0.4500 | g | ||
|
|
ZnSO4 x 7 H2O | 0.2300 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.0970 | g | ||
|
|
CuSO4 x 5 H2O | 0.0100 | g | ||
|
|
CoSO4 x 7 H2O | 0.0056 | g | ||
| 1 Make up to 1 litre with distilled water and adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen. | |||||