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DSMZ

CCAP 40 . JM

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* Medium for Dermamoeba algensis CCAP1524/1

plus Tribonema sp. as food; 20 °C; shaded light

Equipment needed:
Final pH: n.d.
Select a strain (optional):

ml
Compound Amount Unit
Ca(NO3)2 x 4 H2O
(4.0 g/200 ml stock solution) 		1 ml
KH2PO4
(2.48 g/200 ml stock solution) 		1 ml
MgSO4 x 7 H2O
(10.0 g/200 ml stock solution) 		1 ml
NaHCO3
(3.18 g/200 ml stock solution) 		1 ml
Solution 1 1 ml
Solution 2 1 ml
Solution 3 1 ml
NaNO3
(16.0 g/200 ml stock solution) 		1 ml
Na2HPO4 x 12 H2O
(7.2 g/200 ml stock solution) 		1 ml
Deionized water 1000 ml
Agar , for solid medium 15 g
1 Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.

Solution 1 #

200
Compound Amount Unit
FeNa-EDTA 0.45 g
Na2-EDTA 0.45 g

Solution 2 #

200
Compound Amount Unit
H3BO3 0.496 g
MnCl2 x 4 H2O 0.278 g
(NH4)6Mo7O24 x 4 H2O 0.200 g

Solution 3 #

200
Compound Amount Unit
Cyanocobalamine 0.008 g
Thiamine HCl 0.008 g
Biotine 0.008 g