CCAP 63 . NCL

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium C76 500 ml - -
Cereal leaves
(Cerophyll)
1 g 1 -
Deionized water fill up to 1000 ml - -
Medium C76 500 ml - -
1 Make up 1 litre of PJ (Main sol. C 76) with deionized water. Bring 500 ml of PJ (Main sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool to room temperature then restore volume to 500 ml with deionized water. Filter through GF/C paper and decant back into the rest of the PJ (Main sol. C 76). Mix well and autoclave at 15 psi for 15 minutes.
Solution occurs in 13 other media (first defined in Medium C76).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Stock solution (CaCl2/KCl) 1 ml - -
K2HPO4
(0.51 g/100 ml stock solution)
1 ml - -
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution)
1 ml - -
Deionized water 1000 ml - -
1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
Solution occurs in 13 other media (first defined in Medium C76).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.43 g 4.3 29.249
KCl 0.16 g 1.6 21.462

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: New Cereal Leaf-Prescott Liquid
Medium type: Complex medium
Final pH: n.d.
Edit in Medium builder

Associated strains

All strains for this medium: 56
Strains with modifications: 50

Media similar to medium C63

C69 NPA
C76 PJ

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cereal leaves 1 -
CaCl2 x 2 H2O 0.002 0.015
KCl 8.0e-4 0.011
Deionized water - -
K2HPO4 - -
Show more