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CCAP 64 . NCL:MP

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C64 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NCL (New Cereal Leaf-­Prescott Liquid) 750 ml - -
Medium C57 250 ml - -
1 3:1 mixture
2 See separate recipes. Autoclave separately. Mix aseptically when cool.

NCL (New Cereal Leaf-­Prescott Liquid) #

1000
Solution occurs in 3 other media (first defined in Medium C64).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium C76 1000 ml - -
Cereal leaves
(Cerophyll) 		1 g 1 -
Deionized water 1000 ml - -
1 Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.

Medium C76 #

1000
Solution occurs in 13 other media (first defined in Medium C76).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Stock solution (CaCl2/KCl) 1 ml - -
K2HPO4
(0.51 g/100 ml stock solution) 		1 ml - -
MgSO4 x 7 H2O
(0.28 g/100 ml stock solution) 		1 ml - -
Deionized water 1000 ml - -
1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Stock solution (CaCl2/KCl) #

100
Solution occurs in 13 other media (first defined in Medium C76).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.43 g 4.3 29.249
KCl 0.16 g 1.6 21.462

Medium C57 #

1000
Solution occurs in 3 other media (first defined in Medium C57).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(20 g/100 ml stock solution) 		1 ml - -
MgSO4 x 7 H2O
(2 g/100 ml stock solution) 		1 ml - -
Na2HPO4 x 2 H2O
(2 g/100 ml stock solution) 		1 ml - -
KCl
(2.6 g/100 ml stock solution) 		1 ml - -
Stock solution 1 1 ml - -
Deionized water 1000 ml - -
1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Stock solution 1 #

100
Solution occurs in 3 other media (first defined in Medium C57).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe SO4 x 7 H2O 0.2 g 2 7.194
H2SO4
(concentrated) 		0.1 ml - -

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Cereal leaves 0.75 -
CaCl2 x 2 H2O 0.003 0.022
Fe SO4 x 7 H2O 5.0e-4 0.002
Deionized water - -
Na2HPO4 x 2 H2O - -
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