1012 .
ACIDITHRIX FERROOYDANS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
UBS solution | 100.0 | ml | ||
|
|
Trace minerals | 10.0 | ml | ||
|
|
Ni-Se-W solution | 10.0 | ml | ||
|
|
Glucose | 0.9 | g | ||
|
|
Yeast extract | 0.2 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components thoroughly, adjust pH 2.5 with H2SO4 and autoclave. After cooling, add the following solution (filter-sterilized) to the medium: | |||||
|
|
FeSO4
(pH 2.0, 1 M) |
5.0 | ml | ||
UBS solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2SO4 | 14.00 | g |
|
|
(NH4)2SO4 | 30.00 | g |
|
|
KCl | 1.00 | g |
|
|
MgSO4 x 7 H2O | 5.00 | g |
|
|
K2HPO4 | 0.50 | g |
|
|
Ca(NO3)2 x 4 H2O | 0.14 | g |
|
|
Distilled water | 1000.00 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Ni-Se-W solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NiCl2 x 6 H2O | 25.0 | mg |
|
|
(NH4)2Ni(SO4)2 x 6 H2O | 2.0 | g |
|
|
Na2SeO3 | 0.3 | mg |
|
|
Na2SeO4 | 10.0 | mg |
|
|
Na2WO4 x 2 H2O | 10.0 | mg |
|
|
Distilled water | 1000.0 | ml |