1065 .
ACIDIFIED MPOB MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 0.30 | g | ||
|
|
Na2SO4 | 1.42 | g | ||
|
|
CaCl2 x 2 H2O | 0.11 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
NaCl | 0.30 | g | ||
|
|
FeCl2 solution | 1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Yeast extract
(BD-Difco) |
0.20 | g | ||
|
|
Trace vitamins | 10.00 | ml | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly, bubble with a N2-CO2 (80:20, v/v) gas mixture, seal with a butyl rubber stopper and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (separately autoclaved under a N2 atmosphere): | |||||
|
|
K2HPO4
(1 M) |
5.00 | ml | ||
|
|
glycerin
(1 M) |
5.00 | ml | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | ||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |