JCM 1191 . ALKALINE TRYPTICASE SOY BROTH AGAR

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Equipment needed:

Final pH: 10.0

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ml
Compound Amount Unit
Trypticase soy broth
(BD-BBL)
30 g
Agar 15 g
Distilled water 900 ml
1 After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.