Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
CaCl2 x 2 H2O | 0.12 | g | ||
|
MgSO4 x 7 H2O | 0.19 | g | ||
|
KH2PO4 | 0.45 | g | ||
|
K2HPO4 | 0.45 | g | ||
|
NaCl | 0.90 | g | ||
|
(NH4)2SO4 | 0.90 | g | ||
|
Yeast extract
(BD-Difco) |
2.00 | g | ||
|
Trypticase peptone | 2.00 | g | ||
|
n-Butyric acid | 0.40 | ml | ||
|
iso-Butyric acid | 0.40 | ml | ||
|
DL-2-Methylbutyric acid | 0.20 | ml | ||
|
n-Valeric acid | 0.20 | ml | ||
|
iso-Valeric acid | 0.20 | ml | ||
|
Resazurin
(0.05%) |
1.00 | ml | ||
|
L-Cysteine HCl x H2O | 1.00 | g | ||
|
Na2CO3 | 2.50 | g | ||
|
Glucose | 2.00 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Dissolve ingredients (except cysteine, carbonate and glucose), adjust pH to 7.0 and sparge medium with 100% CO2 gas for 30-45 min to make it anoxic. Add cysteine and carbonate, then equilibrate the medium with 100% CO2 gas to pH 7.0. Distribute medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Thereafter, add glucose from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary. |