1239 .
THERMOPLASMA KAMCHATKENSIS MEDIUM
What is this page?
Equipment needed:
Autoclave
Final pH:
2.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
Yeast extract | 0.10 | g | ||
|
|
Sucrose | 5.00 | g | ||
|
|
Sulfur
(powder) |
4.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components except sulfur, yeast extract and sucrose, adjust pH to 2.5 with 10 N H2SO4 and autoclave. Separately autoclave yeast extract and sucrose and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) containing appropriate amount of sulfur, replace gas phase with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. | |||||