J1254
1254 .
HALOARCHAEAL COMPLEX MEDIUM PH 9.0
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.05 | g | - | - | |
|
Fish peptone
(Fluka) |
0.25 | g | - | - | |
| Sodium formate | 0.20 | g | - | - | |
| Sodium acetate | 0.20 | g | - | - | |
| Sodium lactate | 0.20 | g | - | - | |
| Sodium pyruvate | 0.20 | g | - | - | |
| Sodium succinate | 0.20 | g | - | - | |
| Sodium malate | 0.20 | g | - | - | |
| Sodium fumarate | 0.20 | g | - | - | |
| Sodium citrate | 0.20 | g | - | - | |
| KCl | 5.40 | g | - | - | |
| K2HPO4 | 0.20 | g | - | - | |
| CaCl2 x H2O | 0.10 | g | - | - | |
| NaNO3 | 0.50 | g | - | - | |
| MgCl2 x 6 H2O | 0.10 | g | - | - | |
| NaCl | 184.00 | g | - | - | |
| 1 Dissolve components in distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574). After autoclaving, adjust pH to 9.0 by the addition of 10% (w/v) Na2CO3 solution (autoclaved). | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 9.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |