1258 .
DESULFOBACTERIUM OLEOVORANS MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2SO4 | 4.00 | g | ||
|
|
KH2PO4 | 0.20 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
NaCl | 20.00 | g | ||
|
|
MgCl2 x 6 H2O | 3.40 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
FeCl2 solution | 1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 gas atmosphere; **filter-sterilized and stored under an 80% N2-20% CO2 gas mixture): | |||||
|
|
Trace vitamins
(*) |
10.00 | ml | ||
|
|
Vitamin B12 solution 0.05 g/L
(*) |
1.00 | ml | ||
|
|
Steric solution
(see below) |
10.00 | ml | ||
|
|
NaHCO3
(**, 8%) |
20.00 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
8.00 | ml | ||
Stearic solution #
11
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Stearic acid | 0.36 | g | ||
|
|
NaOH
(2 N) |
0.63 | ml | ||
|
|
Distilled water | 10.00 | ml | ||
| 1 Heat the solution in a closed vial under a N2 atmosphere in a boiling water bath. Shake until stearic acid is dissolved and autoclave. Add the solution to the medium while stearic acid is dissolved. Re-melt if stearic acid is solidified. | |||||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.4 | g |
|
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |