159 .
THERMOCOCCUS CELER MEDIUM
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Selected strain: Thermococcus celer DSM 2476
This strain uses the standard medium without modifications.
Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
5.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Brock's salt base solution | 1000.0 | ml | ||
|
|
NaCl | 40.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
2.0 | g | ||
|
|
Sulfur
(powder) |
10.0 | g | ||
|
|
Na2S x 9 H2O | 0.5 | g | ||
|
|
Resazurin | 1.0 | mg | ||
| 1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, adjust pH to 5.8 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 5.8, if necessary. | |||||
Modified Brock's salt base solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g |
|
|
KH2PO4 | 0.28 | g |
|
|
MgSO4 x 7 H2O | 0.25 | g |
|
|
CaCl2 x 2 H2O | 0.07 | g |
|
|
FeCl3 x 6 H2O | 2.00 | mg |
|
|
MnCl2 x 4 H2O | 1.80 | mg |
|
|
Na2B4O7 x 10 H2O | 4.50 | mg |
|
|
ZnSO4 x 7 H2O | 0.22 | mg |
|
|
CuCl2 x 2 H2O | 0.05 | mg |
|
|
Na2MoO4 x 2 H2O | 0.03 | mg |
|
|
VOSO4 x n H2O | 0.03 | mg |
|
|
CoSO4 x 7 H2O | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |