215 .
PYROBACULUM AEROPHILUM MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Marine medium/Synthetic seawater mix solution | 125.00 | ml | ||
|
|
Trace minerals | 10.00 | ml | ||
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 2.00 | mg | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
(NH4)2Ni(SO4)2 x 6 H2O | 2.00 | mg | ||
|
|
Na2SeO4 | 0.10 | mg | ||
|
|
Na2WO4 x 2 H2O | 0.10 | mg | ||
|
|
NaHCO3 | 2.20 | g | ||
|
|
KH2PO4 | 0.07 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.50 | g | ||
|
|
Na2S2O3 x 5 H2O | 1.00 | g | ||
|
|
Distilled water | 865.00 | ml | ||
| 1 Adjust pH to 7.0 with 1 N H2SO4, and sterilize by filtration. Dispense the medium into bottles under a N2-CO2-O2 (80:20:1) atmosphere. Pressurize inoculated bottles to 200 kPa N2-CO2-O2 (80:20:1). | |||||
Marine medium/Synthetic seawater mix solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 47.15 | g |
|
|
MgCl2 x 6 H2O | 18.10 | g |
|
|
MgSO4 x 7 H2O | 7.00 | g |
|
|
CaCl2 x 2 H2O | 3.13 | g |
|
|
Na2SO4 | 3.24 | g |
|
|
Na2CO3 | 0.10 | g |
|
|
NaBr | 0.10 | g |
|
|
KBr | 80.00 | mg |
|
|
SrCl2 x 6 H2O | 72.00 | mg |
|
|
H3BO3 | 52.00 | mg |
|
|
Na2HPO4 | 8.10 | mg |
|
|
NaF | 2.40 | mg |
|
|
Sodium silicate | 0.40 | mg |
|
|
KI | 0.05 | mg |
|
|
Distilled water | 1000.00 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||