279 .
HYDROGENOPHILUS HIRSCHII MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 7.00 | g | ||
|
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MgCl2 x 6 H2O | 5.50 | g | ||
|
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KCl | 0.65 | g | ||
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NaBr | 0.10 | g | ||
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NaHCO3 | 2.00 | g | ||
|
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NH4Cl | 0.15 | g | ||
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CaCl2 x 2 H2O | 0.50 | g | ||
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Trace minerals | 10.00 | ml | ||
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(NH4)2Ni(SO4)2 x 6 H2O | 0.02 | g | ||
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Na2WO4 x 2 H2O | 0.10 | mg | ||
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Na2SeO4 | 0.10 | mg | ||
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Na2S2O3 x 5 H2O | 1.00 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0 with H2SO4. Distribute 20 ml of medium into 120 ml serum bottles under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Autoclave and stand the bottles overnight. Add 15 ml filter-sterilized air and pressurize the gas phase to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture. | |||||
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
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MgSO4 x 7 H2O | 3.00 | g | ||
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MnSO4 x n H2O | 0.50 | g | ||
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NaCl | 1.00 | g | ||
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FeSO4 x 7 H2O | 0.10 | g | ||
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CoSO4 x 7 H2O | 0.10 | g | ||
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CaCl2 x 2 H2O | 0.10 | g | ||
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ZnSO4 x 7 H2O | 0.10 | g | ||
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CuSO4 x 5 H2O | 0.01 | g | ||
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AlK(SO4)2 | 0.01 | g | ||
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H3BO3 | 0.01 | g | ||
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Na2MoO4 x 2 H2O | 0.01 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||