335 .
JCM MEDIUM No. 335
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Equipment needed:
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 0.78 | g | ||
|
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KH2PO4 | 0.75 | g | ||
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Na3-EDTA | 0.04 | g | ||
|
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FeSO4 x H2O | 0.01 | g | ||
|
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MgSO4 x H2O | 0.25 | g | ||
|
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CaCl2 x H2O | 0.03 | g | ||
|
|
NaCl | 0.20 | g | ||
|
|
NH4Cl | 27.00 | mg | ||
|
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Na2SO4 | 2.80 | g | ||
|
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Sodium acetate | 0.16 | g | ||
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Trace vitamins | 10.00 | ml | ||
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Trace element solution | 10.00 | ml | ||
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L-Cysteine HCl x H2O | 0.25 | g | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 5.5. Fill the gas phase with H2-CO2 (80:20). | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
|
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
|
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Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.800 | g | ||
|
|
FeCl3 x 6 H2O | 1.350 | g | ||
|
|
MnCl2 x 4 H2O | 0.100 | g | ||
|
|
CoCl2 x 6 H2O | 0.024 | g | ||
|
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CaCl2 x 2 H2O | 0.100 | g | ||
|
|
ZnCl2 | 0.100 | g | ||
|
|
CuCl2 x 2 H2O | 0.025 | g | ||
|
|
H3BO3 | 0.010 | g | ||
|
|
NaCl | 1.000 | g | ||
|
|
NiCl2 x 6 H2O | 0.120 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.026 | g | ||
|
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Distilled water | 1000.000 | ml | ||
| 1 Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH. | |||||
| 2 Comment: For strain JCM 10134, incubate with a 10-20% inoculum. | |||||