336 .
PE MEDIUM (pH 6.8)
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Equipment needed:
Final pH:
6.8
Select a strain (optional):
Main sol. J303 #
1011
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Basal salts solution | 5.0 | ml | ||
|
|
Phosphate solution | 5.0 | ml | ||
|
|
Vitamin solution | 1.0 | ml | ||
|
|
(NH4)2SO4 | 0.5 | g | ||
|
|
Na glutamate | 0.5 | g | ||
|
|
Sodium succinate | 0.5 | g | ||
|
|
Sodium acetate | 0.5 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.5 | g | ||
|
|
Casamino acids
(BD-Difco) |
0.5 | g | ||
|
|
Na2S2O3 | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.5. | |||||
Basal salts solution #
1010
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na3-EDTA | 4.12 | g |
|
|
FeSO4 x H2O | 1.11 | g |
|
|
MgSO4 x 7 H2O | 24.65 | g |
|
|
CaCl2 x 2 H2O | 2.94 | g |
|
|
NaCl | 23.40 | g |
|
|
Trace elements solution | 10.00 | ml |
|
|
Distilled water | 1000.00 | ml |
Vitamin solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Nicotinic acid | 100 | mg |
|
|
Thiamine HCl | 100 | mg |
|
|
Biotin | 5 | mg |
|
|
p-Aminobenzoic acid | 50 | mg |
|
|
Vitamin B12 | 1 | mg |
|
|
Calcium pantothenate | 50 | mg |
|
|
Pyridoxine hydrochloride | 50 | mg |
|
|
Folic acid | 50 | mg |
|
|
Na3-EDTA | 200 | mg |
|
|
Distilled water | 100 | ml |