347 .
SME MEDIUM
What is this page?
Equipment needed:
Autoclave
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Synthetic seawater (2 x)
(2 x) |
500.0 | ml | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
(NH4)2SO4 | 0.5 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.4 | g | ||
|
|
Sulfur
(powder) |
10.0 | g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Na2S x 9 H2O | 0.5 | g | ||
|
|
Distilled water | 500.0 | ml | ||
| 1 Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, and autoclave. Separately autoclave 10% (w/v) yeast extract solution, and 5% Na2S x 9H2O solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, add the yeast extract solution, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v) gas mixture. | |||||
Synthetic seawater (2 x) #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 55.40 | g |
|
|
MgSO4 x 7 H2O | 14.00 | g |
|
|
MgCl2 x 6 H2O | 11.00 | g |
|
|
KCl | 1.30 | g |
|
|
NaBr | 0.20 | g |
|
|
H3BO3 | 0.06 | g |
|
|
SrCl2 x 6 H2O | 0.03 | g |
|
|
KI | 0.10 | mg |
|
|
Trisodium citrate | 20.00 | mg |
|
|
CaCl2 x 2 H2O | 1.50 | g |
|
|
Distilled water | 1000.00 | ml |