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JCM 384 . GLYCEROL-SOIL MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J384 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone
(BD-Difco) 		5 g 5 -
Beef extract
(BD-Difco) 		3 g 3 -
Glycerol 20 g 20 217.179
Soil extract
(See below) 		150 ml - -
Tap water 850 ml - -
Agar 15 g 15 44.599
1 Adjust pH to 7.0.

Soil extract #

Equipment needed: Filter Autoclave
1 Suspend 400 g of air-dried garden soil in 960 ml of tap water and autoclave at 121C for 1 hr. After the mixture is cooled and settled, the supernatant is decanted, filtered through paper, autoclaved, and stored at room temperature until cleared by sedimentation.

Metadata

Last modified: 24.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycerol 20 217.179
Agar 15 44.599
Bacto peptone 5 -
Beef extract 3 -
Soil extract - -
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