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MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT
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Selected strain: Mycobacterium abscessus subsp. bolletii DSM 45149
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Middlebrook 7H10 agar
(BD-Difco) |
19 | g | ||
|
|
Glycerol | 5 | ml | ||
|
|
Distilled water | 895 | ml | ||
| 1 Sterilize at 121C for 10 min and cool to 50 - 55C. Aseptically add 100 ml of Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly. | |||||