417 .
MJYTFSH MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 20.00 | g | ||
|
|
K2HPO4 | 0.90 | g | ||
|
|
KH2PO4 | 0.70 | g | ||
|
|
CaCl2 x 2 H2O | 1.00 | g | ||
|
|
NH4Cl | 1.25 | g | ||
|
|
MgSO4 x 7 H2O | 3.40 | g | ||
|
|
MgCl2 x 6 H2O | 4.18 | g | ||
|
|
KCl | 0.33 | g | ||
|
|
NiCl2 x 6 H2O | 5.00 | mg | ||
|
|
Na2SeO3 x 5 H2O | 5.00 | mg | ||
|
|
Fe2(SO4)3 x n H2O | 0.01 | g | ||
|
|
H2WO4 | 5.00 | mg | ||
|
|
FeSO4 x 7 H2O | 0.02 | g | ||
|
|
Yeast extract
(BD-Difco) |
1.00 | g | ||
|
|
Tryptone
(BD-Difco) |
1.00 | g | ||
|
|
Sodium formate | 0.20 | g | ||
|
|
Trace mineral solution | 10.00 | ml | ||
|
|
Trace vitamins | 1.00 | ml | ||
|
|
NaHCO3 | 2.00 | g | ||
|
|
Sulfur
(powder) |
10.00 | g | ||
|
|
Na2S x 9 H2O | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix ingredients, except Trace vitamins, NaHCO3 and sulfur, adjust pH to 5.5, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sterilized sulfur under a stream of H2-CO2 (4:1, v/v) gas mixture, sparge for 5 min with the same gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add the sterilized Na2S x 9H2O solution to the medium, and pressurize to 200 kPa H2-CO2 (4:1, v/v). Readjust pH to 5.5, if necessary. | |||||
Trace mineral solution #
| 1 Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI. |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |