438 .
RT2 MEDIUM
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Equipment needed:
Autoclave
Filter
Hungate tubes
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 1.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
KH2PO4 | 0.10 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
Yeast extract | 0.50 | g | ||
|
|
CAPSO | 11.86 | g | ||
|
|
Na2SO4 | 4.00 | g | ||
|
|
Trace element solution
(see Medium No. 294) |
1.00 | ml | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
|
Sodium lactate | 2.24 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Sodium dithionite | 0.10 | g | ||
| 1 Dissolve components except sodium lactate and sodium dithionite in 900 ml distilled water and adjust pH to 9.0 with NaOH. Bring to a boil, and cool down under a N2 gas stream. Distribute the medium into culture vessels (e.g., 9 ml of the medium in Hungate tubes) under N2, seal with butyl rubber stoppers and autoclave. Separately autoclave 0.2 M sodium lactate under a N2 atmosphere. After cooling, add 1/9 volume of the sodium lactate solution to the medium. Readjust pH to 9.0, if necessary. Prior to use, reduce the medium with a freshly prepared, filter-sterilize 1% sodium dithionite solution. | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.4 | g |
|
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
|
Distilled water | 1000.0 | ml |