Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Agar
(Sigma, reinforced clostridial) |
51 | g | ||
|
Distilled water | 900 | ml | ||
1 After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0. |