459 .
CAPNOCYTOPHAGA MEDIUM
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Equipment needed:
Autoclave
Final gas composition:
10 % CO2
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Final pH:
7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone
(BD-BBL) |
17 | g | ||
|
|
Yeast extract
(BD-Difco) |
3 | g | ||
|
|
Glucose | 3 | g | ||
|
|
NaCl | 3 | g | ||
|
|
KNO3 | 3 | g | ||
|
|
Hemin | 3 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||
| 2 Autoclave glucose solution separately. Incubate under an oxygen-free atmosphere containing 5-10% CO2. | |||||