514 .
GLUCOSE SULFIDE MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 0.15 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
(NH4)2SO4 | 0.50 | g | ||
|
|
CaCO3 | 0.10 | g | ||
|
|
Ca(NO3)2 | 0.10 | g | ||
|
|
KCl | 0.05 | g | ||
|
|
K2HPO4 | 0.05 | g | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
Na2S x 9 H2O | 0.20 | g | ||
|
|
Vitamin solution | 10.00 | ml | ||
|
|
Agar | 15.00 | g | ||
|
|
Distilled water | 990.00 | ml | ||
| 1 Add components except Vitamin solution to distilled water and bring volume to 990 ml. Adjust pH to 7.3. After autoclaving, aseptically add filter-sterilized Vitamin solution. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.00 | mg |
|
|
Folic acid | 2.00 | mg |
|
|
Pyridoxine hydrochloride | 10.00 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.00 | mg |
|
|
Riboflavin | 5.00 | mg |
|
|
Nicotinic acid | 5.00 | mg |
|
|
Calcium pantothenate | 5.00 | mg |
|
|
p-Aminobenzoic acid | 1.00 | mg |
|
|
Myo-inositol | 1.00 | mg |
|
|
Vitamin B12 | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |