779 .
MODIFIED HALOANAEROBIUM MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 75.0 | g | ||
|
|
MgSO4 x 7 H2O | 8.8 | g | ||
|
|
KCl | 1.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
Trypticase peptone
(BD-BBL) |
10.0 | g | ||
|
|
Trace vitamins | 10.0 | ml | ||
|
|
Trace minerals | 10.0 | ml | ||
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH 8.0 with NaOH. Autoclave under a N2 atmosphere. After cooling, add anaerobically the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
Glucose
(10%) |
25.0 | ml | ||
|
|
Thioglycolate-ascorbate solution
(*) |
25.0 | ml | ||
| 2 Readjust pH to 8.0, if necessary. | |||||
Thioglycolate-ascorbate solution #
25
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sodium thioglycolate | 0.5 | g | ||
|
|
Sodium ascorbate | 0.5 | g | ||
|
|
Distilled water | 25.0 | ml | ||
| 1 Prepare just before use. Filter-sterilize. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||