825 .
KOKO MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone
(BD-Difco) |
1.00 | g | ||
|
|
Peptone | 1.00 | g | ||
|
|
Yeast extract | 1.00 | g | ||
|
|
K2HPO4 | 1.60 | g | ||
|
|
NaH2PO4 x 2 H2O | 1.00 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.16 | g | ||
|
|
CaCl2 x 2 H2O | 0.06 | g | ||
|
|
Trace element solution SL-4 | 10.00 | ml | ||
|
|
Trace vitamins | 10.00 | ml | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Distilled water | 930.00 | ml | ||
| 1 Mix components thoroughly and adjust pH to 7.0. Autoclave the medium under a N2 atmosphere. Add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
|
Glucose
(10%) |
50.00 | ml | ||
|
|
NaHCO3
(*, 8%) |
1.25 | ml | ||
|
|
L-Cysteine HCl x H2O
(3%) |
10.00 | ml | ||
|
|
Na2S x 9 H2O
(3%) |
10.00 | ml | ||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 0.5 | g |
|
|
FeSO4 x 7 H2O | 0.2 | g |
|
|
Trace element solution SL-6 | 100.0 | ml |
|
|
Distilled water | 900.0 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |