974 .
ALKALINUS ABYSSINIESE MEDIUM
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 117.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
5.0 | g | ||
|
|
Peptone
(Oxoid) |
5.0 | g | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
CaCl2 x 2 H2O | 0.2 | g | ||
|
|
KH2PO4 | 0.1 | g | ||
|
|
Trace mineral solution | 10.0 | ml | ||
| 1 Add components to distilled water and bring volume to 900 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add aseptically the following solutions (separately autoclaved): | |||||
|
|
Glucose
(w/v, 10%) |
5.0 | ml | ||
|
|
Na2CO3
(w/v, 10%) |
100.0 | ml | ||
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
H3BO4 | 30 | mg |
|
|
ZnSO4 x 7 H2O | 10 | mg |
|
|
Na2MoO4 x 2 H2O | 3 | mg |
|
|
MnCl2 x 4 H2O | 3 | mg |
|
|
NiCl2 x 6 H2O | 2 | mg |
|
|
CoCl2 x 6 H2O | 10 | mg |
|
|
CuCl2 x 2 H2O | 1 | mg |
|
|
Distilled water | 1000 | ml |