989 .
IRD AMINOBACTERIUM MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 1.0 | g | ||
|
|
KH2PO4 | 0.3 | g | ||
|
|
K2HPO4 | 0.3 | g | ||
|
|
NaCl | 20.0 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
|
L-Serine | 1.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
2.0 | g | ||
|
|
Trace element solution | 1.0 | ml | ||
|
|
Selenite-tungstate solution | 1.0 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Resazurin | 0.5 | mg | ||
|
|
Distilled water | 945.0 | ml | ||
| 1 Mix components thoroughly and adjust pH to 7.2. Bring to a boil, cool down under a N2-CO2 (4:1, v/v) gas stream, seal with butyl rubber stoppers and autoclave. Aseptically and anaerobically add the following stock solutions [autoclaved and stored under a N2 atmosphere or *filter-sterilize and store under a N2-CO2 (4:1, v/v) gas mixture]: | |||||
|
|
MgCl2 x 6 H2O
(15%) |
20.0 | ml | ||
|
|
NaHCO3
(*, 8%) |
25.0 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
10.0 | ml | ||
| 2 Readjust pH to 7.2, if necessary. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%) |
12.5 | ml |
|
|
FeSO4 x 7 H2O | 2.1 | g |
|
|
H3BO3 | 30.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 987.0 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.4 | g |
|
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
|
Distilled water | 1000.0 | ml |