995 .
M30
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Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
Solution 1 #
1030
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Hutner's basal salts | 20 | ml | ||
|
|
Sea water
(aged) |
700 | ml | ||
|
|
Tris-HCl buffer
(pH 7.5, 0.1 M) |
50 | ml | ||
|
|
Distilled water | 260 | ml | ||
| 1 For solid medium, add 5.0 g/L gelrite (Gellan Gum). | |||||
Solution 2 #
54
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
N-Acetyl-D-glucosamine
(Sigma) |
2.00 | g | ||
|
|
Na2HPO4 x 2 H2O | 0.01 | g | ||
|
|
Ampicillin
(50 mg/ml) |
4.00 | ml | ||
|
|
Vitamin solution No. 6 | 10.00 | ml | ||
|
|
Distilled water | 40.00 | ml | ||
| 1 Add 0.2 ml filter-sterilized Solution 2 to Solution 1. | |||||
Vitamin solution No. 6 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Biotin | 4.0 | mg | ||
|
|
Pyridoxine hydrochloride | 20.0 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 10.0 | mg | ||
|
|
Calcium pantothenate | 10.0 | mg | ||
|
|
p-Aminobenzoic acid | 10.0 | mg | ||
|
|
Folic acid | 4.0 | mg | ||
|
|
Riboflavin | 10.0 | mg | ||
|
|
Nicotinamide | 10.0 | mg | ||
|
|
Vitamin B12 | 0.2 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Filter-sterilize. | |||||
Modified Hutner's basal salts #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | ||
|
|
MgSO4 x 7 H2O | 29.700 | g | ||
|
|
CaCl2 x 2 H2O | 3.335 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 9.250 | mg | ||
|
|
FeSO4 x 7 H2O | 99.000 | mg | ||
|
|
Metals ''44'' | 50.000 | ml | ||
|
|
Distilled water | 950.000 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4. | |||||