Strain modifications for Medium 1

100 distinct modifications were found.

Adjust pH of final medium to 7.0.

Modified strains

Arthrobacter alpinus DSM 22274
Cytobacillus gottheilii DSM 23668

Annotated modifications

7.0

Adjust pH of final medium to 6.8 - 7.0.

Modified strains

Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 22625
Anoxybacillus salavatliensis DSM 22626
Geobacillus thermodenitrificans subsp. calidus DSM 22628
Geobacillus thermodenitrificans subsp. calidus DSM 22629

Annotated modifications

6.8 - 7.0

Add 1% glucose

Modified strains

Pseudomonas amygdali DSM 7298

Annotated modifications

10.0 g/l Glucose

Add 1% methanol

Modified strains

Methylopila capsulata DSM 6130
Methylophilus methylotrophus DSM 6330
Methylovorus glucosotrophus DSM 6874
Methylobacillus flagellatus DSM 6875
Methylobacillus glycogenes DSM 46233
Methylobacillus glycogenes DSM 46234
Microvirga flocculans DSM 15743

Annotated modifications

10.0 g/l Methanol

Add 1% methanol

Modified strains

Methylorubrum podarium DSM 15083

Annotated modifications

10.0 g/l Methanol

Add 3% NaCl

Modified strains

Halomonas venusta DSM 7049

Annotated modifications

30.0 g/l NaCl

Addition of vitamins for example see DSMZ medium 461) enhances reactivation and growth

Modified strains

Erwinia tracheiphila DSM 21139

develops better in liquid medium

Modified strains

Aneurinibacillus thermoaerophilus DSM 10154

Annotated modifications

Agar

Diluted 1:10, plus 1 g/l glucose

Modified strains

Elstera litoralis DSM 19532

Annotated modifications

1.0 g/l Glucose

Grows on agar plates better than in liquid medium

Modified strains

Stenotrophomonas ginsengisoli DSM 24757

Growth on solid medium

Modified strains

Paenibacillus curdlanolyticus DSM 10247
Paenibacillus curdlanolyticus DSM 10248
Paenibacillus kobensis DSM 10250

Incubate under 10% (v/v) O2, 90% N2

Modified strains

Cupriavidus necator DSM 430

Annotated modifications

10% O2, 90% N2

Liquid

Modified strains

Sphingobium xenophagum DSM 14677

Annotated modifications

Agar

Liquid medium for reactivation of freeze-dried culture

Modified strains

Ottowia konkukae DSM 105395

Annotated modifications

Agar

Liquid, with 20 % horse serum

Modified strains

Streptobacillus moniliformis DSM 12112

Annotated modifications

200.0 g/l Horse serum
Agar

No growth, only for rehydration of freeze-dryed material

Modified strains

Xylella taiwanensis DSM 103350

One fifth diluted

Modified strains

Paenibacillus tyrfis DSM 100708

Only liquid

Modified strains

Fluviicola taffensis DSM 16823

Annotated modifications

Agar

Optimum 28°C

Modified strains

Chryseobacterium gregarium DSM 19109

Plus 0,5 - 1 % methanol

Modified strains

Methylobacterium thuringiense DSM 23631
Methylobacterium sp. DSM 23632

Annotated modifications

15.0 g/l Methanol

Plus 0.1% Na-acetate

Modified strains

Blastomonas ursincola DSM 9006

Annotated modifications

1.0 g/l Na-acetate

Plus 0.1% urea

Modified strains

Paenibacillus agarexedens DSM 1327
Paenibacillus sp. DSM 1352
Paenibacillus sp. DSM 1354
Paenibacillus agaridevorans DSM 1355
Paenibacillus sp. DSM 1356
Paenibacillus sp. DSM 1358
Paenibacillus sp. DSM 1474
Paenibacillus agarexedens DSM 1475
Paenibacillus agarexedens DSM 1477
Paenibacillus agarexedens DSM 1478
Paenibacillus agarexedens DSM 1479
Paenibacillus sp. DSM 1480
Paenibacillus sp. DSM 1481
Paenibacillus sp. DSM 1482
Paenibacillus agaridevorans DSM 1483
Paenibacillus agaridevorans DSM 1484
Paenibacillus agaridevorans DSM 1485
Paenibacillus agaridevorans DSM 1486

Annotated modifications

1.0 g/l Urea

Plus 0.2% urea

Modified strains

Paenibacillus agarexedens DSM 1473

Annotated modifications

2.0 g/l Urea

Plus 0.2% yeast extract

Modified strains

Brevibacillus agri DSM 8372
Brevibacillus parabrevis DSM 8376

Annotated modifications

2.0 g/l Yeast extract

Plus 0.5% NaCl

Modified strains

Flavobacterium frigidimaris DSM 15937

Annotated modifications

5.0 g/l NaCl

Plus 1% glucose

Modified strains

Shinella yambaruensis DSM 18801

Annotated modifications

10.0 g/l Glucose

Plus 1% MeOH

Modified strains

Methylopila jiangsuensis DSM 22718

Annotated modifications

10.0 g/l MeOH

Plus 1% methanol

Modified strains

Methylorubrum extorquens DSM 1337
Methylorubrum extorquens DSM 1338
Methylorubrum extorquens DSM 1339
Methylorubrum extorquens DSM 1340
Methylobacillus sp. DSM 1758
Methylorubrum rhodesianum DSM 1759
Methylobacillus sp. DSM 1760
Methylobacterium radiotolerans DSM 1819
Methylobacterium sp. DSM 1857
Methylorubrum rhodinum DSM 2163
Methylorubrum aminovorans DSM 8832
Methylorubrum thiocyanatum DSM 11490
Methylorubrum extorquens DSM 13060
Methylobacterium phyllosphaerae DSM 19779

Annotated modifications

10.0 g/l Methanol

Plus 10 g/l NaCl

Modified strains

Mongoliibacter ruber DSM 27929

Annotated modifications

10.0 g/l NaCl

Plus 10 mg/l MnSO4

Modified strains

Paenibacillus elgii DSM 22254
Paenibacillus donghaensis DSM 22278
Bacillus cytotoxicus DSM 22905
Geobacillus uzenensis DSM 23175

Annotated modifications

10.0 mg/l MnSO4

Plus 10 mg/L MnSO4; pH 6.0 - 8.0

Modified strains

Bacillus velezensis DSM 23117

Annotated modifications

10 mg/L MnSO4
6.0 - 8.0

Plus 10 mg/L MnSO4; pH 7.0

Modified strains

Bacillus sp. DSM 23473
Bacillus sp. DSM 23474

Annotated modifications

10 mg/L MnSO4
7.0

Plus 10 mg/L MnSO4; pH 8.0

Modified strains

Anoxybacillus tengchongensis DSM 23211
Anoxybacillus eryuanensis DSM 23212

Annotated modifications

10 mg/L MnSO4
8.0

Plus 10 µg/ml tetracycline

Modified strains

Cupriavidus necator DSM 3102

Annotated modifications

10.0 mg/l Tetracycline

Plus 10% NaCl

Modified strains

Halobacillus karajensis DSM 14948
Planococcus rifietoensis DSM 15069

Annotated modifications

100.0 g/l NaCl

Plus 10% NaCl; pH 7.5

Modified strains

Roseivivax sediminis DSM 21977

Annotated modifications

100.0 g/l NaCl
7.5

Plus 10mg/l MnSO4

Modified strains

Anoxybacillus kamchatkensis subsp. asaccharedens DSM 18475

Annotated modifications

10.0 mg/l MnSO4

Plus 20 mg/l uracil

Modified strains

Pseudomonas putida DSM 26234
Pseudomonas putida DSM 26250
Pseudomonas putida DSM 26251
Pseudomonas putida DSM 26252
Pseudomonas putida DSM 26253

Annotated modifications

20.0 mg/l Uracil

Plus 200 mg/l NiCl2

Modified strains

Bacillus licheniformis DSM 7459

Annotated modifications

200.0 mg/l NiCl2

Plus 3% NaCl

Modified strains

Virgibacillus proomii DSM 13055

Annotated modifications

30.0 g/l NaCl

Plus 4 g NaCl/L

Modified strains

Serratia marcescens DSM 14187

Annotated modifications

4.0 g NaCl

Plus 5 g/l methanol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 plus 5 g/l methanol. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Albibacter helveticus DSM 22800

Annotated modifications

5.0 g/l Methanol

Plus 5 g/l NaCl

Modified strains

Lysobacter ruishenii DSM 22393
Lysobacter xinjiangensis DSM 25188
Gibbsiella dentisursi DSM 27944
Chelatococcus thermostellatus DSM 28244

Annotated modifications

5.0 g/l NaCl

Plus 5 g/l NaCl; Reactivate and grow freeze-dried cells from the ampoule in liquid medium 1 or 365. Subsequent subculturing may be carried out in liquid medium or on agar.

Modified strains

Vogesella indigofera DSM 3303

Annotated modifications

5.0 g/l NaCl

Plus 50% soil extract (see medium 80 or 12 for preparation)

Modified strains

Paenibacillus castaneae DSM 19417

Annotated modifications

500.0 g/l Soil extract

Plus 500 µg/ml streptomycin

Modified strains

Cupriavidus necator DSM 15444

Annotated modifications

500.0 mg/l Streptomycin

plus 0.1% Na2CO3

Modified strains

Annotated modifications

0.1 % Na2CO3

plus 0.1% urea

Modified strains

Annotated modifications

0.1 % Urea

Plus 0.5% NaCl

Modified strains

Photorhabdus laumondii DSM 107756

Annotated modifications

5.0 g/l NaCl

Plus 1% MeOH

Modified strains

Methylobacterium sp. DSM 12291

Annotated modifications

10.0 g/l MeOH

plus 1% methanol

Modified strains

Annotated modifications

1 % Methanol

plus 10 mg/L MnSO4

Modified strains

Annotated modifications

10 mg/l MnSO4

plus 10 µg/ml MnSO4

Modified strains

Annotated modifications

10 mg/l MnSO4

plus 100 g/L NaCl

Modified strains

Annotated modifications

100 g/l NaCl

plus 2% NaCl

Modified strains

Annotated modifications

2 % NaCl

Plus 4% NaCl

Modified strains

Virgibacillus proomii DSM 2242

Annotated modifications

40.0 g/l NaCl

Plus 5 g/l NaCl

Modified strains

Ancylobacter defluvii DSM 29296

Annotated modifications

5.0 g/l NaCl

Plus 5 g/l NaCl plus 1 % (v/v) MeOH

Modified strains

Methylopila oligotropha DSM 29295

Annotated modifications

5.0 g/l NaCl
1.0 %(v/v) Methanol

plus 50 µg/ml thiamine

Modified strains

Annotated modifications

50 mg/L Thiamine

Plus 50% soil extract

Modified strains

Cytobacillus firmus DSM 12
Paenibacillus anaericanus DSM 15890

Annotated modifications

500.0 g/l Soil extract

Plus 50% soil extract and 5% NaCl

Modified strains

Virgibacillus halodenitrificans DSM 10037
Acerihabitans sp. DSM 104038

Annotated modifications

500.0 g/l Soil extract
5 % NaCl

plus Manganese sulfate (10 µg/ml)

Modified strains

Psyhrotrophic, cultivate below 25 °C

Modified strains

Janthinobacterium svalbardensis DSM 25734

Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Lysobacter capsici DSM 23109

Rehydrate and grow freeze dried cells of ampoule in liquid medium 1. Subsequent subculturing may be carried out in liquid media or on agar plates.

Modified strains

Xanthomonas albilineans DSM 3583

Rehydrate and grow freeze-dried material from the ampoule in liquid medium 1. Subsequent subculturing may be carried out on medium 1 agar plates or in mineral medium with salicylate.

Modified strains

Pseudomonas moorei DSM 12647

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 535. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Dickeya paradisiaca DSM 18069

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Rhodococcus sp. DSM 6427
Xanthomonas campestris DSM 3586
Sphingobium chlorophenolicum DSM 7098
Pseudomonas marginalis DSM 50275

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Chitinimonas taiwanensis DSM 18899
Xanthomonas hyacinthi DSM 50855

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Xanthomonas vesicatoria DSM 50861

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid or agar medium 1 or on agar plates of medium 585

Modified strains

Bosea minatitlanensis DSM 13099

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates, in medium 1 or on.

Modified strains

Caballeronia zhejiangensis DSM 28073

Rehydrate and grow lyophilized cells from the ampoule in liquid broth 1 or 54. Subsequently, subculturing may be carried out in liquid medium or on agar plates

Modified strains

Xanthomonas hortorum DSM 50857

Rehydrate and grow lyophilized cells from the ampoule in liquid medium 1 or 381. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Pseudomonas kilonensis DSM 13647

Rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates

Modified strains

Xanthomonas campestris DSM 2405

To reactivate freeze-dried cells, incubate them in liquid medium for one day before inoculating agar media.

Modified strains

Pseudomonas savastanoi DSM 50298

With 0.2% glucose

Modified strains

Mycobacterium sp. DSM 3803

Annotated modifications

2.0 g/l Glucose

With 0.2% pullulan

Modified strains

Bacillus sp. DSM 6834

Annotated modifications

2.0 g/l Pullulan

With 0.4% yeast extract and 0.2% benzoate

Modified strains

Bacillus benzoevorans DSM 5391
Bacillus benzoevorans DSM 5392

Annotated modifications

4.0 g/l Yeast extract
2.0 g/l Na-benzoate

With 0.4% yeast extract and 0.2% sodiumbenzoate

Modified strains

Bacillus sp. DSM 5977
Bacillus sp. DSM 5978
Bacillus sp. DSM 5979
Bacillus sp. DSM 5981

Annotated modifications

4.0 g/l Yeast extract
2.0 g/l Na-benzoate

With 0.5% NaCl

Modified strains

Escherichia coli DSM 8226
Chitinophaga japonensis DSM 13484

Annotated modifications

5.0 g/l NaCl

With 0.8 g/l NaCl

Modified strains

Escherichia coli DSM 5210

Annotated modifications

0.8 g/l NaCl

With 1 % (v/v) methanol

Modified strains

Albibacter methylovorans DSM 22840

Annotated modifications

1.0 %(v/v) Methanol

With 1 mg vitamin B12 (cyanocobalamine)

Modified strains

Deinococcus radiodurans DSM 46620

Annotated modifications

1.0 mg Vitamin B12

With 1% glycerol and 0.4% K- or Na-oxalate

Modified strains

Oxalicibacterium flavum DSM 15507

Annotated modifications

10.0 g/l Glycerol
4.0 g/l Na-oxalate

With 1% glycerol and 0.4% K- or Na-oxalate; rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates

Modified strains

Oxalicibacterium flavum DSM 15506

Annotated modifications

10.0 g/l Glycerol
4.0 g/l Na-oxalate

With 1% v/v methanol

Modified strains

Methylobacterium radiotolerans DSM 760
Methylophilus sp. DSM 1634
Methylobacterium mesophilicum DSM 1708
Methylorubrum extorquens DSM 6343
Methylophilus leisingeri DSM 6813
Methylovorus sp. DSM 11726
Methylobacterium organophilum DSM 18172
Methylobacterium oryzae DSM 18207

Annotated modifications

1.0 %(v/v) Methanol

With 10 mg/L manganese sulfate

Modified strains

Parageobacillus thermoglucosidasius DSM 21625

Annotated modifications

10 mg/L Manganese Sulfate

With 10 mg/L MnSO4

Modified strains

Anoxybacillus kaynarcensis DSM 21706

Annotated modifications

10 mg/L MnSO4

With 25 µg/ml tetracycline

Modified strains

Salmonella enterica subsp. enterica DSM 554

Annotated modifications

25.0 mg/l (-)-Tetracycline

With 4 g/l yeast

Modified strains

Schinkia azotoformans DSM 5811

Annotated modifications

4.0 g/l Yeast

With 4 g/l yeast extract

Modified strains

Schinkia azotoformans DSM 5810

Annotated modifications

4.0 g/l Yeast extract

With 40 µg/ml Thymin

Modified strains

Bacillus subtilis DSM 5214

Annotated modifications

40.0 mg/l Thymine

With 5 g/l NaCl

Modified strains

Collimonas arenae DSM 21398
Collimonas pratensis DSM 21399
Photorhabdus tasmaniensis DSM 22387
Photorhabdus caribbeanensis DSM 22391
Xenorhabdus eapokensis DSM 104079

Annotated modifications

5.0 g/l NaCl

With 5 g/l NaCl; rehydrate freeze dried cells in 5 ml medium 545. Incubate this culture until growth occurs. Subsequently, cultivation may be carried out in medium 1 or medium 545 and on agar plates.

Modified strains

Photorhabdus luminescens DSM 3368

Annotated modifications

5.0 g/l NaCl

With 50 mg/l streptomycin sulfate

Modified strains

Pseudomonas putida DSM 2112

Annotated modifications

50.0 mg/l Streptomycin

With 50% soil extract

Modified strains

Paenibacillus ehimensis DSM 11029
Gracilibacillus dipsosauri DSM 11125
Bacillus sp. DSM 11173
Bacillus sp. DSM 11174
Bacillus sp. DSM 11175
Bacillus sp. DSM 11176

Annotated modifications

500.0 g/l Soil extract

With 50% soil extract and 0.3% yeast extract

Modified strains

Paenibacillus chitinolyticus DSM 11030

Annotated modifications

500.0 g/l Soil extract
3.0 g/l Yeast extract

With 80 IU Penicillin G

Modified strains

Bacillus cereus DSM 6127

Annotated modifications

80 IU Penicillin G

Plus 10% NaCl

Modified strains

Nocardiopsis halotolerans DSM 44410
Actinopolyspora erythraea DSM 45583

Annotated modifications

100.0 g/l NaCl
Modifications
Adjust pH of final medium to 7.0. Adjust pH of final medium to 6.8 - 7.0. Add 1% glucose Add 1% methanol Add 1% methanol Add 3% NaCl Addition of vitamins for example see DSMZ medium 461) enhances reactivation and growth develops better in liquid medium Diluted 1:10, plus 1 g/l glucose Grows on agar plates better than in liquid medium Growth on solid medium Incubate under 10% (v/v) O2, 90% N2 Liquid Liquid medium for reactivation of freeze-dried culture Liquid, with 20 % horse serum No growth, only for rehydration of freeze-dryed material One fifth diluted Only liquid Optimum 28°C Plus 0,5 - 1 % methanol Plus 0.1% Na-acetate Plus 0.1% urea Plus 0.2% urea Plus 0.2% yeast extract Plus 0.5% NaCl Plus 1% glucose Plus 1% MeOH Plus 1% methanol Plus 10 g/l NaCl Plus 10 mg/l MnSO4 Plus 10 mg/L MnSO4; pH 6.0 - 8.0 Plus 10 mg/L MnSO4; pH 7.0 Plus 10 mg/L MnSO4; pH 8.0 Plus 10 µg/ml tetracycline Plus 10% NaCl Plus 10% NaCl; pH 7.5 Plus 10mg/l MnSO4 Plus 20 mg/l uracil Plus 200 mg/l NiCl2 Plus 3% NaCl Plus 4 g NaCl/L Plus 5 g/l methanol; Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 plus 5 g/l methanol. Subsequent subculturing may be carried out in liquid medium or on agar plates Plus 5 g/l NaCl Plus 5 g/l NaCl; Reactivate and grow freeze-dried cells from the ampoule in liquid medium 1 or 365. Subsequent subculturing may be carried out in liquid medium or on agar. Plus 50% soil extract (see medium 80 or 12 for preparation) Plus 500 µg/ml streptomycin plus 0.1% Na2CO3 plus 0.1% urea Plus 0.5% NaCl Plus 1% MeOH plus 1% methanol plus 10 mg/L MnSO4 plus 10 µg/ml MnSO4 plus 100 g/L NaCl plus 2% NaCl Plus 4% NaCl Plus 5 g/l NaCl Plus 5 g/l NaCl plus 1 % (v/v) MeOH plus 50 µg/ml thiamine Plus 50% soil extract Plus 50% soil extract and 5% NaCl plus Manganese sulfate (10 µg/ml) Psyhrotrophic, cultivate below 25 °C Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow freeze dried cells of ampoule in liquid medium 1. Subsequent subculturing may be carried out in liquid media or on agar plates. Rehydrate and grow freeze-dried material from the ampoule in liquid medium 1. Subsequent subculturing may be carried out on medium 1 agar plates or in mineral medium with salicylate. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1 or 535. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth medium 1. Subsequent subculturing may be carried out in liquid or agar medium 1 or on agar plates of medium 585 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 1. Subsequent subculturing may be carried out in liquid medium or on agar plates, in medium 1 or on. Rehydrate and grow lyophilized cells from the ampoule in liquid broth 1 or 54. Subsequently, subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in liquid medium 1 or 381. Subsequent subculturing may be carried out in liquid medium or on agar plates. Rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates To reactivate freeze-dried cells, incubate them in liquid medium for one day before inoculating agar media. With 0.2% glucose With 0.2% pullulan With 0.4% yeast extract and 0.2% benzoate With 0.4% yeast extract and 0.2% sodiumbenzoate With 0.5% NaCl With 0.8 g/l NaCl With 1 % (v/v) methanol With 1 mg vitamin B12 (cyanocobalamine) With 1% glycerol and 0.4% K- or Na-oxalate With 1% glycerol and 0.4% K- or Na-oxalate; rehydrate and grow lyophylisate in liquid medium 1. Subsequently, subculturing may be carried out in liquid medium or on agar plates With 1% v/v methanol With 10 mg/L manganese sulfate With 10 mg/L MnSO4 With 25 µg/ml tetracycline With 4 g/l yeast With 4 g/l yeast extract With 40 µg/ml Thymin With 5 g/l NaCl With 5 g/l NaCl; rehydrate freeze dried cells in 5 ml medium 545. Incubate this culture until growth occurs. Subsequently, cultivation may be carried out in medium 1 or medium 545 and on agar plates. With 50 mg/l streptomycin sulfate With 50% soil extract With 50% soil extract and 0.3% yeast extract With 80 IU Penicillin G Plus 10% NaCl