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DSMZ

Strain modifications for Medium 104

63 distinct modifications were found.

Adjust pH of final medium to 7.0.

Modified strains

Parafannyhessea umbonata DSM 22619
Parafannyhessea umbonata DSM 22620

Annotated modifications

7.0

(add 0.5 g/l Tween 80); anaerobic

Modified strains

Bulleidia extructa DSM 13220

Annotated modifications

0.5 g/l Tween 80

Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, 0.1% soluble starch, and 2% rumen fluid

Modified strains

Actinomyces ruminicola DSM 116972

Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch; anaerobic

Modified strains

Actinomyces massiliensis DSM 23047
Actinomyces oris DSM 23056

Annotated modifications

4.0 g/l Glucose
1.0 g/l Cellobiose
1.0 g/l Maltose
1.0 g/l Starch

Anaerobic (90% N2, 5% H2, 5% CO2)

Modified strains

Leptotrichia shahii DSM 19757
Leptotrichia wadei DSM 19758

Annotated modifications

90% N2, 5% H2, 5% CO2

Anaerobic, N2CO2 plus H2 (1 bar)

Modified strains

Waltera intestinalis DSM 108985

Anaerobic, 100% CO2 atmosphere

Modified strains

Tonsilliphilus suis DSM 21880

Annotated modifications

100% CO2

dilute the medium 1:10

Modified strains

Coprobacillus tertius DSM 114538

dilute the medium 1:100

Modified strains

Allocoprobacillus halotolerans DSM 114537

Plus 3g/L cellubiose

Modified strains

Actinotalea fermentans DSM 3133

Annotated modifications

3 g/L Cellobiose

Plus 0.05% mucin

Modified strains

Akkermansia glycaniphila DSM 100705

Annotated modifications

0.5 g/l Mucin

Plus 0.1% mucin

Modified strains

Akkermansia muciniphila DSM 22959

Annotated modifications

1.0 g/l Mucin

Plus 0.25% Sodium lactate

Modified strains

Veillonella tobetsuensis DSM 104750

Annotated modifications

2.5 g/l Sodium lactate

Plus 0.5% fructose and 3% rumen fluid

Modified strains

Monoglobus pectinilyticus DSM 104782

Annotated modifications

5.0 g/l Fructose
30.0 g/l Rumen fluid

Plus 0.5% maltose

Modified strains

Clostridium nitritogenes DSM 3985

Annotated modifications

5.0 g/l Maltose

Plus 1 % rumen fluid and volatile fatty acids (see medium 330); anaerobic

Modified strains

Denitrobacterium detoxificans DSM 21845

Annotated modifications

10.0 g/l Rumen fluid
+ 10 ml Volatile fatty acid mixture

Plus 1 g/l arginine

Modified strains

Collinsella tanakaei DSM 22478

Annotated modifications

1.0 g/l Arginine

Plus 1% rumen fluid and volatile fatty acids (see medium 330)

Modified strains

Denitrobacterium detoxificans DSM 21825

Annotated modifications

10.0 g/l Rumen fluid
+ 10 ml Volatile fatty acid mixture

Plus 10µl/ml bovine serum

Modified strains

Fusobacterium equinum DSM 17476

Annotated modifications

10.0 ml/l Bovine serum

Plus 10µl/ml putrescine (0.03%) plus 10 µl/ml sodium lactate (50%)

Modified strains

Veillonella montpellierensis DSM 17217

Annotated modifications

10 µl/ml Putrescine
10 µl/ml Sodium lactate

Plus 15% rumen fluid

Modified strains

Actinomyces ruminicola DSM 27982

Annotated modifications

150.0 g/l Rumen fluid

Plus 3 g/l arginine

Modified strains

Slackia piriformis DSM 22477

Annotated modifications

3.0 g/l Arginine

Plus 5% fetal calf serum

Modified strains

Buchananella hordeovulneris DSM 20732

Annotated modifications

50.0 g/l Calf serum

Plus 5% horse serum

Modified strains

Prevotella micans DSM 21469

Annotated modifications

50.0 g/l Horse serum

Plus 5% horse serum plus bicarbonate

Modified strains

Mobiluncus curtisii subsp. curtisii DSM 23059

Annotated modifications

50.0 g/l Horse serum

Plus arginine (1g/L)

Modified strains

Collinsella tanakaei DSM 22514

Annotated modifications

1.0 g/l Arginine

Plus bovine serum (10µl/ml)

Modified strains

Actinomyces oricola DSM 17234
Helcococcus sueciensis DSM 17243

Annotated modifications

10.0 ml/l Bovine serum

Plus bovine serum (10µl/ml)

Modified strains

Propionibacterium acidifaciens DSM 21887

Annotated modifications

10.0 ml/l Bovine serum

Plus carbohydrate-mix (g/L): Glucose 4.0, cellobiose 1.0, maltose 1.0, starch (soluble) 1.0.; strictly anaerobic

Modified strains

Faecalicoccus pleomorphus DSM 26962

Annotated modifications

4.0 g/l Glucose
1.0 g/l Cellobiose
1.0 g/l Maltose
1.0 g/l Starch

Plus disodium succinate 2.5g/l

Modified strains

Peptostreptococcus sp. DSM 9536

Annotated modifications

2.5 g/l Disodium succinate

Plus fetal bovine serum (1%)

Modified strains

Peptostreptococcus stomatis DSM 17678

Annotated modifications

10.0 g/l Fetal bovine serum

Plus fructose (2 g/L); anaerobic

Modified strains

Megasphaera micronuciformis DSM 17226

Annotated modifications

2.0 g/l Fructose

Plus haemine (50µg/ml) plus Vit K1 plus Na-lactate (0.5%)

Modified strains

Veillonella magna DSM 109050

Annotated modifications

50.0 mg/l Haemin
5.0 g/l Na-lactate

Plus haemine (5µg/ml) plus Vit K1

Modified strains

Eubacterium ventriosum DSM 3988
Granulicatella adiacens DSM 9848
Fusobacterium nucleatum subsp. polymorphum DSM 20482
Gemella morbillorum DSM 20572
Actinomyces viscosus DSM 43327
Segatella oris DSM 18711
Eubacterium nodatum DSM 3993
Alistipes timonensis DSM 25383
Actinotignum sanguinis DSM 26039
Megasphaera massiliensis DSM 26228

Annotated modifications

5.0 mg/l Haemin

Plus haemine (5µg/ml) plus Vit K1

Modified strains

Cutibacterium avidum DSM 4901
Bacteroides fragilis DSM 9668
Phocaeicola vulgatus DSM 9670
Prevotella nigrescens DSM 13386
Fusobacterium nucleatum subsp. nucleatum DSM 15643
Finegoldia magna DSM 20362
Cutibacterium granulosum DSM 20458
Prevotella denticola DSM 20614
Cutibacterium granulosum DSM 20700
Prevotella intermedia DSM 20706
Alloscardovia omnicolens DSM 21503
Fusobacterium necrophorum subsp. necrophorum DSM 21784
Peptoniphilus senegalensis DSM 25694
Prevotella melaninogenica DSM 26980
Actinomyces israelii DSM 43011
Fannyhessea vaginae DSM 15829
Parabacteroides distasonis DSM 108211
Eisenbergiella tayi DSM 108217
Faecalicatena contorta DSM 108236
Eubacterium tenue DSM 108237
Cutibacterium modestum DSM 109769

Annotated modifications

5.0 mg/l Haemin

Plus haemine (5µg/ml) plus Vit K1 plus Na-lactate (0.75%)

Modified strains

Veillonella seminalis DSM 111052

Annotated modifications

7.5 g/l Na-lactate
5.0 mg/l Haemin

Plus haemine (5µg/ml) plus Vit K1; obligately anaerobic

Modified strains

Drancourtella massiliensis DSM 100440

Annotated modifications

5.0 mg/l Haemin

Plus haemine (5µg/ml) plus Vit K1; strictly anaerobic

Modified strains

Holdemania massiliensis DSM 26143

Annotated modifications

5.0 mg/l Haemin

Plus haemine plus Vit K 3; anaerobic

Modified strains

Odoribacter laneus DSM 22474

Plus haemine plus Vit K1 plus lactate (0.75%)

Modified strains

Phascolarctobacterium succinatutens DSM 22533

Annotated modifications

7.5 g/l Lactate

Plus horse serum (5% vol/vol)

Modified strains

Leyella stercorea DSM 18206

Annotated modifications

5.0 %(v/v) Horse serum

Plus horse serum (5%) plus NaHCO3 (0.1%)

Modified strains

Mobiluncus mulieris DSM 25311

Annotated modifications

50.0 g/l Horse serum
1.0 g/l NaHCO3

Plus L-Arginin (1g/l)

Modified strains

Adlercreutzia equolifaciens subsp. equolifaciens DSM 19450

Annotated modifications

1.0 g/l L-Arginine

Plus L-Arginine 1.0 g/L and sterile fetal bovine serum (100 µl/10 ml); anaerobic

Modified strains

Slackia exigua DSM 15923

Annotated modifications

1.0 g/l L-Arginine
10.0 ml/l Fetal bovine serum

Plus lactate (0.5 - 1%); anaerobic

Modified strains

Veillonella rogosae DSM 18960
Veillonella magna DSM 19857

Annotated modifications

7.5 g/l Lactate

Plus lactate (0.75%) plus putrescine (3 mg/l)

Modified strains

Veillonella parvula subsp. parvula DSM 2008

Annotated modifications

7.5 g/l Lactate
3.0 mg/l Putrescine

Plus Na-lactate (10 g/l)

Modified strains

Propionicicella superfundia DSM 22317

Annotated modifications

10.0 g/l Na-lactate

Plus rumen fluid (200µl/10ml); anaerobic

Modified strains

Bacteroides coprosuis DSM 18011

Annotated modifications

20.0 ml/l Rumen fluid

plus 0.1% mucin

Modified strains

Akkermansia biwaensis DSM 114407

plus 0.5 g/L pectin

Modified strains

Collinsella sp. DSM 109235

plus 5% bovine serum

Modified strains

Scardovia wiggsiae DSM 22547

plus haemin plus Vit K1

Modified strains

Neobacillus muris DSM 110989

plus haemin plus VitK plus horse serum (5%)

Modified strains

Mobiluncus mulieris DSM 2710

Annotated modifications

5 % Horse serum

plus haemine (5µg/ml) plus Vit K1

Modified strains

Parvimonas micra DSM 20468
Schaalia hyovaginalis DSM 10695
Arachnia propionica DSM 43015
Fusobacterium perfoetens DSM 117056

Annotated modifications

5 mg/l Haemin

plus haemine plus Vit K1

Modified strains

Muricaecibacterium torontonense DSM 110159
Streptococcus caecimuris DSM 110150
Weizmannia agrestimuris DSM 106041

Plus Na-lactate (0.75%)

Modified strains

Phascolarctobacterium succinatutens DSM 22521

Annotated modifications

7.5 g/l Na-lactate

plus vitamin solution (see medium 141)

Modified strains

Bacteroides propionicifaciens DSM 19346

Supplement medium after autoclaving with 50.0 ml/l clarified rumen fluid (see medium 1310), 50.0 ml/l horse serum (Bio & Sell PFD.SE.0500) and 0.2 g/l dithiothreitol (DTT) added from filter-sterilized anoxic stock solutions.

Modified strains

Acetatifactor muris DSM 23669
Fretibacterium fastidiosum DSM 25557

Annotated modifications

50.0 ml/l Clarified rumen fluid
0.2 g/l Dithiothreitol
50.0 ml/l Horse serum

Supplemented with arginine (2g/L); strictly anaerobic

Modified strains

Slackia heliotrinireducens DSM 20476

Annotated modifications

2.0 g/l Arginine

Supplemented with rumenfluid (30 ml/L)

Modified strains

Ruthenibacterium lactatiformans DSM 100348

Annotated modifications

30.0 ml/l Rumen fluid

Supplemented with sodium lactate (10g/L); strictly anaerobic

Modified strains

Acidipropionibacterium virtanenii DSM 106790

Annotated modifications

10.0 g/l Sodium lactate

With 1 g/l of NaHCO3; anaerobic

Modified strains

Anaerobiospirillum succiniciproducens DSM 6400

Annotated modifications

1.0 g/l NaHCO3

With carbohydrates (see medium 110); anaerobic

Modified strains

Anaerobutyricum hallii DSM 3353
Modifications
Adjust pH of final medium to 7.0. (add 0.5 g/l Tween 80); anaerobic Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, 0.1% soluble starch, and 2% rumen fluid Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch; anaerobic Anaerobic (90% N2, 5% H2, 5% CO2) Anaerobic, N2CO2 plus H2 (1 bar) Anaerobic, 100% CO2 atmosphere dilute the medium 1:10 dilute the medium 1:100 Plus 3g/L cellubiose Plus 0.05% mucin Plus 0.1% mucin Plus 0.25% Sodium lactate Plus 0.5% fructose and 3% rumen fluid Plus 0.5% maltose Plus 1 % rumen fluid and volatile fatty acids (see medium 330); anaerobic Plus 1 g/l arginine Plus 1% rumen fluid and volatile fatty acids (see medium 330) Plus 10µl/ml bovine serum Plus 10µl/ml putrescine (0.03%) plus 10 µl/ml sodium lactate (50%) Plus 15% rumen fluid Plus 3 g/l arginine Plus 5% fetal calf serum Plus 5% horse serum Plus 5% horse serum plus bicarbonate Plus arginine (1g/L) Plus bovine serum (10µl/ml) Plus bovine serum (10µl/ml) Plus carbohydrate-mix (g/L): Glucose 4.0, cellobiose 1.0, maltose 1.0, starch (soluble) 1.0.; strictly anaerobic Plus disodium succinate 2.5g/l Plus fetal bovine serum (1%) Plus fructose (2 g/L); anaerobic Plus haemine (50µg/ml) plus Vit K1 plus Na-lactate (0.5%) Plus haemine (5µg/ml) plus Vit K1 Plus haemine (5µg/ml) plus Vit K1 Plus haemine (5µg/ml) plus Vit K1 plus Na-lactate (0.75%) Plus haemine (5µg/ml) plus Vit K1; obligately anaerobic Plus haemine (5µg/ml) plus Vit K1; strictly anaerobic Plus haemine plus Vit K 3; anaerobic Plus haemine plus Vit K1 plus lactate (0.75%) Plus horse serum (5% vol/vol) Plus horse serum (5%) plus NaHCO3 (0.1%) Plus L-Arginin (1g/l) Plus L-Arginine 1.0 g/L and sterile fetal bovine serum (100 µl/10 ml); anaerobic Plus lactate (0.5 - 1%); anaerobic Plus lactate (0.75%) plus putrescine (3 mg/l) Plus Na-lactate (10 g/l) Plus rumen fluid (200µl/10ml); anaerobic plus 0.1% mucin plus 0.5 g/L pectin plus 5% bovine serum plus haemin plus Vit K1 plus haemin plus VitK plus horse serum (5%) plus haemine (5µg/ml) plus Vit K1 plus haemine plus Vit K1 Plus Na-lactate (0.75%) plus vitamin solution (see medium 141) Supplement medium after autoclaving with 50.0 ml/l clarified rumen fluid (see medium 1310), 50.0 ml/l horse serum (Bio & Sell PFD.SE.0500) and 0.2 g/l dithiothreitol (DTT) added from filter-sterilized anoxic stock solutions. Supplemented with arginine (2g/L); strictly anaerobic Supplemented with rumenfluid (30 ml/L) Supplemented with sodium lactate (10g/L); strictly anaerobic With 1 g/l of NaHCO3; anaerobic With carbohydrates (see medium 110); anaerobic