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104. PYG MEDIUM (MODIFIED)

Culture medium recipe

Vol.
×

M 104 #

ml
Solution occurs in 1 other media (first defined in medium 104 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.0 g 4.995 -
2 5.0 g 4.995 -
3 10.0 g 9.99 -
4 5.0 g 4.995 -
5 5.0 g 4.995 27.726
6 2.0 g 1.998 11.471
7 1.0 ml - -
8 Salt solution 40.0 ml - -
9 1.0 mg 1.0e-3 0.004
10 Vitamin K1 solution 0.2 ml - -
11 Haemin solution 10.0 ml - -
12 0.5 g 0.5 2.844
13 950.0 ml 947.349 52585.8
14 , required for pH adjustment
(8 N)
x - -
17
(optional)
0.2 g/l 0.2 1.052
Instructions:
  1. The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
7 1000.00 ml 998.207 55408.8
Solution occurs in 2 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50 mg 0.495 -
2
(1 N)
1 ml 21.089 527.266
3 100 ml 988.324 54860.2
Instructions:
  1. Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated.

SL 104: Vitamin K1 solution #

20
Solution occurs in 2 other media (first defined in medium 104 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2 0.1 ml - -
3
(95 %)
20.0 ml 750.5 16291
Instructions:
  1. Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 416
Strains with modifications: 394
104 PYG MEDIUM (MODIFIED)
a ANAEROBACULUM MEDIUM
b PY + X MEDIUM
c PY + X MEDIUM (N2/CO2)
d MALEDIVIBACTER MEDIUM (BETAINE)

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 997.111 55348
Yeast extract 9.99 -
Glucose 4.995 27.726
Beef extract 4.995 -
Peptone 4.995 -
Trypticase peptone 4.995 -
K2HPO4 2.038 11.701
L-Cysteine HCl x H2O 0.5 2.844
NaHCO3 0.4 4.757
NaOH 0.211 5.267
Dithiothreitol 0.2 1.052
Ethanol 0.15 3.255
KH2PO4 0.04 0.294
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Haemin 0.005 -
Resazurin 1.0e-3 0.004
Tween 80 - -
Vitamin K1 - -

Cultivation metadata from 416 strains BacDive

halophily not 12 strains
halophily slightly 7 strains
halophily moderate 5 strains
pH 6 - 8 33 strains
pH 4 - 6 22 strains
pH 8 - 10 17 strains
pH 2 - 4 1 strain
pH 10 - 14 1 strain
phyla 8 391 strains
nutrition chemoorganotroph 1 strain
nutrition chemoorganoheterotroph 1 strain
oxygen anaerobe 299 strains
oxygen microaerophile 37 strains
oxygen obligate anaerobe 22 strains
oxygen facultative anaerobe 16 strains
oxygen aerobe 3 strains
oxygen aerotolerant 2 strains
temperature mesophilic 387 strains
temperature thermophilic 11 strains
temperature psychrophilic 1 strain

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